Extraction of polyphenols associated with pectin from olive waste (alperujo) with choline chloride

被引:21
作者
Bermudez-Oria, Alejandra [1 ]
Fernandez-Prior, Africa [1 ]
Castejon, Maria Luisa [1 ]
Rodriguez-Gutierrez, Guillermo
Fernandez-Bolanos, Juan [1 ]
机构
[1] CSIC, Spanish Natl Res Council, Dept Food Phytochem, Inst Grasa, Ctra Utrera Km 1,Pablo de Olavide Univ Campus,Bldg, Seville 41013, Spain
关键词
Pectin-polyphenols complex; Choline chloride; Natural deep eutectic solvent; NMR and IR spectroscopy; Antioxidant activity; Caco-2 cell line; DEEP EUTECTIC SOLVENTS; POLYSACCHARIDES; TOXICITY; PRODUCTS; WATER;
D O I
10.1016/j.foodchem.2023.136073
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
The main by-product from olive oil extraction (alperujo) was extracted with hot water, citric acid, natural deep eutectic solvent (choline chloride: citric acid), and only choline chloride. The purified extracts were composed of macromolecular complexes constituting polyphenols associated with pectin. The extracts were structurally characterized by FT-IR and solid-NMR spectroscopy and an in vitro test revealed distinct antioxidant and antiproliferative activity, depending on the extracting agents. The choline chloride-extracted complex contained the highest amount of polyphenols among the examined agents, which exhibited a strong antioxidant activity and significant antiproliferative capacity. However, the complex extracted by hot water showed the highest antiproliferative capacity in vitro against the colon carcinoma Caco-2 cell line. In this finding, choline chloride could be used as a novel, green and promising alternative to the conventional extracting agent for the production of complexes that combine the antioxidant activity of phenolic compounds and the physiological effects of pectic polysaccharides.
引用
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页数:10
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