Fish waste valorization;
EPA and DHA;
Enzymatic extraction;
Design of experiments;
Life cycle analysis;
LIFE-CYCLE ASSESSMENT;
EXTRACTION;
PROTEIN;
OIL;
NITROGEN;
QUALITY;
YIELD;
LCA;
D O I:
10.1016/j.fbp.2022.11.006
中图分类号:
Q81 [生物工程学(生物技术)];
Q93 [微生物学];
学科分类号:
071005 ;
0836 ;
090102 ;
100705 ;
摘要:
Tuna represents one of the most consumed foods in many parts of the world. It is esti-mated that about 70 % of the tuna's weight is discarded in the production of canned fillets. This work is focused on the optimization of production of an oil rich in omega-3 from the tuna viscera, provided by a canned tuna production company, using the Alcalase enzyme. Combined use of Design of experiments (DoE), life cycle analysis (LCA), and principal component analysis (PCA) on the collected analytical data made it possible to define the best combination of the values of pH, enzyme/substrate (E/S) ratio, and reaction time, respectively (pH = 8.5), E/S (1 %), and time (120 min), and to better understand the en-vironmental bottleneck of the process. The outcomes of the study demonstrated that the duration of the hydrolysis and the associated electricity consumption is the factor that affects the most the environmental sustainability of the process.(c) 2022 Institution of Chemical Engineers. Published by Elsevier Ltd. All rights reserved.