Prognostic role of macrophages and mast cells in the microenvironment of hepatocellular carcinoma after resection

被引：5

作者：

Ali, Esraa ^{[1
]}

Cervenkova, Lenka ^{[2
,3
]}

Palek, Richard ^{[2
,4
,5
]}

Ambrozkiewicz, Filip ^{[1
]}

Hosek, Petr ^{[2
]}

Daum, Ondrej ^{[6
,7
]}

Liska, Vaclav ^{[2
,4
,5
]}

Hemminki, Kari ^{[1
,8
]}

Trailin, Andriy ^{[1
]}

机构：

[1] Charles Univ Prague, Fac Med Pilsen, Biomed Ctr, Lab Translat Canc Genom, Alej Svobody 1655-76, Plzen 32300, Czech Republic

[2] Charles Univ Prague, Fac Med Pilsen, Biomed Ctr, Lab Canc Treatment & Tissue Regenerat, Alej Svobody 1655-76, Plzen 32300, Czech Republic

[3] Charles Univ Prague, Fac Med 3, Dept Pathol, Ruska 87, Prague 10000, Czech Republic

[4] Charles Univ Prague, Fac Med Pilsen, Dept Surg, Alej Svobody 80, Plzen 32300, Czech Republic

[5] Charles Univ Prague, Fac Med Pilsen, Biomed Ctr, Alej Svobody 80, Plzen 32300, Czech Republic

[6] Charles Univ Prague, Fac Med & Teaching Hosp Plzen, Sikls Inst Pathol, Edvarda Benese 13, Plzen 30599, Czech Republic

[7] Biopticka Lab Sro, Mikulasske Nam 4, Plzen 32600, Czech Republic

[8] German Canc Res Ctr, Dept Canc Epidemiol, Neuenheimer Feld 280, D-69120 Heidelberg, Germany

来源：

BMC CANCER | 2024年 / 24卷 / 01期

关键词：

Hepatocellular carcinoma; Tumor-infiltrating macrophages; Mast cells; Inner margin; Peritumor area; Disease-free survival; TUMOR-INFILTRATING MACROPHAGES; POLARIZATION; CANCER; RECURRENCE; SURVIVAL; DENSITY; GROWTH; STROMA; CD68; M1;

D O I：

10.1186/s12885-024-11904-8

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

BackgroundThe prognostic significance of mast cells and different phenotypes of macrophages in the microenvironment of hepatocellular carcinoma (HCC) following resection is unclear. We aimed in this study to assess the local distribution of infiltrating macrophages and mast cells of specific phenotypes in tissues of HCC and to evaluate their prognostic values for survival of post-surgical patients.MethodsThe clinicopathological and follow-up data of 70 patients with HCC, who underwent curative resection of tumor from 1997 to 2019, were collected. The infiltration of CD68+ and CD163+ macrophages and CD117+ mast cells was assessed immunohistochemically in representative resected specimens of HCC and adjacent tissues. The area fraction (AF) of positively stained cells was estimated automatically using QuPath image analysis software in several regions, such as tumor center (TC), inner margin (IM), outer margin (OM), and peritumor (PT) area. The prognostic significance of immune cells, individually and in associations, for time to recurrence (TTR), disease-free survival (DFS), and overall survival (OS) was evaluated using Kaplan-Meier and Cox regression analyses.ResultsHigh AF of CD68+ macrophages in TC and IM and high AF of mast cells in IM and PT area were associated with a longer DFS. High AF of CD163+ macrophages in PT area correlated with a shorter DFS. Patients from CD163TChigh & CD68TClow group had a shorter DFS compared to all the rest of the groups, and cases with CD163IMlow & CD68IMhigh demonstrated significantly longer DFS compared to low AF of both markers. Patients from CD68IMhigh & CD163PTlow group, CD117IMhigh & CD163PTlow group, and CD117PThigh & CD163PTlow group had a significantly longer DFS compared to all other combinations of respective cells.ConclusionsThe individual prognostic impact of CD68+ and CD163+ macrophages and mast cells in the microenvironment of HCC after resection depends on their abundance and location, whereas the cumulative impact is built upon combination of different cell phenotypes within and between regions.

引用

页数：13

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