Tetraclinis articulata (Vahl) Mast. essential oil as a promising source of bioactive compounds with antimicrobial, antioxidant, anti-inflammatory and dermatoprotective properties: In vitro and in silico evidence

被引：14

作者：

El Hachlafi, Naoufal ^{[1
]}

Fikri-Benbrahim, Kawtar ^{[1
]}

Al-Mijalli, Samiah Hamad ^{[2
]}

Elbouzidi, Amine ^{[3
,4
]}

Jeddi, Mohamed ^{[1
]}

Abdallah, Emad M. ^{[5
]}

Assaggaf, Hamza ^{[6
]}

Bouyahya, Abdelhakim ^{[7
]}

Alnasser, Sulaiman Mohammed ^{[8
]}

Attar, Ammar ^{[6
]}

Goh, Khang Wen ^{[9
]}

Ming, Long Chiau ^{[10
]}

Ong, Seng-Kai ^{[10
]}

Mrabti, Hanae Naceiri ^{[11
,12
]}

Chahdi, Fouad Ouazzani ^{[12
]}

机构：

[1] Sidi Mohamed Ben Abdellah Univ, Sci & Technol Fac, Lab Microbial Biotechnol & Bioact Mol, Imouzzer Rd,POB 2202, Fes, Morocco

[2] Princess Nourah Bint Abdulrahman Univ, Coll Sci, Dept Biol, POB 84428,17, Riyadh 11671, Saudi Arabia

[3] Univ Mohammed Premier, Fac Sci, Lab Ameliorat Prod Agr & Biotechnol Environm LAPAB, Oujda 60000, Morocco

[4] Euro Mediterranean Univ Fes UEMF, Fes, Morocco

[5] Qassim Univ, Coll Sci & Arts, Dept Sci Labs, Ar Rass 51921, Saudi Arabia

[6] Umm Al Qura Univ, Fac Appl Med Sci, Dept Lab Med, Mecca 21955, Saudi Arabia

[7] Mohammed V Univ, Fac Sci, Dept Biol, Lab Human Pathol Biol, Rabat 10106, Morocco

[8] Qassim Univ, Unaizah Coll Pharm, Dept Pharmacol & Toxicol, Qasim 51452, Saudi Arabia

[9] INTI Int Univ, Fac Data Sci & Informat Technol, Nilai 71800, Malaysia

[10] Sunway Univ, Sch Med & Life Sci, Sunway City, Malaysia

[11] High Inst Nursing Profess & Hlth Tech, Casablanca, Morocco

[12] Sidi Mohammed Ben Abdellah Univ, Fac Sci & Technol Fez, Lab Appl Organ Chem, Fes, Morocco

来源：

HELIYON | 2024年 / 10卷 / 01期

关键词：

Tetraclinis articulata; Molecular docking; Biological activities; GC-MS profiling; Microbial resistance; CHEMICAL-COMPOSITION; ANTIBACTERIAL; INFLAMMATION; MECHANISMS; EXTRACTION; TYROSINASE; PROTEIN; ACIDS;

D O I：

10.1016/j.heliyon.2023.e23084

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Tetraclinis articulata is a known traditional medicinal plant used to manage various ailments, such as diabetes, rheumatism and infectious diseases. This study aims to determine the chemical constituents of T. articulata essential oil (EO) and to evaluate its in vitro antibacterial, anticandidal, antioxidant, anti-inflammatory and dermatoprotective properties. In addition, a computational docking approach was used to predict the potential antioxidant, antibacterial, antifungal, anti-inflammatory, and cytotoxic properties of the identified compounds. The volatile oil obtained by hydrodistillation was characterized using gas chromatography-mass spectrometry (GC-MS). The antioxidant activity of T. articulata EO was investigated using three complementary assays: DPPH, ABTS and FRAP. Lipoxygenase (5-LOX) and tyrosinase enzymes were used to assess the anti-inflammatory and dermatoprotective effects of this oil. Moreover, disc-diffusion technique, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) assays were employed for the antimicrobial screening. The GC-MS analysis revealed that bornyl acetate (41.80 %), alpha-pinene (17.97 %) and camphor (15.97 %) are the major components of the studied EO. Moreover, T. articulata EO has exhibited promising antioxidant effect on FRAP, DPPH, and ABTS experiments. It also significantly inhibited 5-LOX (IC50 = 67.82 +/- 0.03 mu g/mL) and tyrosinase (IC50 = 211.93 +/- 0.02 mu g/mL). The results of MIC and MBC assays indicated that T. articulata EO is able to inhibit the growth of all tested bacteria (Gram + and Gram -) and Candida species. The ratio of tolerance level indicated that the tested oil was bactericidal against the Gram + bacteria and Candida species, whereas it has a bacteriostatic behavior against the Gram(-) bacteria. In light of these findings, T. articulata EO may be suggested as a potential pharmaceutical agent to prevent inflammation and skin problems and may serve as a natural antimicrobial and antioxidant alternative for sustainable application in food products.

引用

页数：17

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