Identifying a dynamic transcriptomic landscape of the cynomolgus macaque placenta during pregnancy at single-cell resolution

被引:7
作者
Jiang, Xiangxiang [1 ,2 ]
Zhai, Jingle [1 ,5 ,7 ]
Xiao, Zhenyu [1 ,3 ,5 ,7 ]
Wu, Xulun [1 ,5 ,7 ]
Zhang, Dan [4 ]
Wan, Haifeng [1 ,5 ,7 ]
Xu, Yanhong [1 ,5 ,6 ]
Qi, Luqing [1 ,5 ,6 ,7 ]
Wang, Meijiao [5 ,7 ]
Yu, Dainan [1 ,5 ,6 ,7 ]
Liu, Yawei [1 ,5 ,7 ]
Wu, Hao [1 ,5 ,7 ]
Sun, Run [1 ,5 ,6 ,7 ]
Xia, Shuwei [1 ,5 ,6 ,7 ]
Yu, Kunyuan [1 ,5 ,6 ,7 ]
Guo, Jingtao [1 ,5 ,6 ,7 ]
Wang, Hongmei [1 ,5 ,6 ,7 ]
机构
[1] Chinese Acad Sci, Inst Zool, State Key Lab Stem Cell & Reprod Biol, Beijing 100101, Peoples R China
[2] Anhui Med Univ, NHC Key Lab Study Abnormal Gametes & Reprod Tract, Hefei 230032, Peoples R China
[3] Beijing Inst Technol, Sch Life Sci, Beijing 100081, Peoples R China
[4] Zhejiang Univ, Key Lab Reprod Genet, Sch Med, Minist Educ,Womens Hosp, Hangzhou 310006, Peoples R China
[5] Chinese Acad Sci, Inst Stem Cell & Regenerat, Beijing 100101, Peoples R China
[6] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
[7] Beijing Inst Stem Cell & Regenerat Med, Beijing 100101, Peoples R China
基金
中国国家自然科学基金;
关键词
RECEPTORS; DIFFERENTIATION; LIGANDS;
D O I
10.1016/j.devcel.2023.03.012
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Supporting healthy pregnancy outcomes requires a comprehensive understanding of the cellular hierarchy and underlying molecular mechanisms in the primate placenta during gestation. Here, we present a single -cell transcriptome-wide view of the cynomolgus macaque placenta throughout gestation. Bioinformatics analyses and multiple validation experiments suggested that placental trophoblast cells exhibited stage -specific differences across gestation. Interactions between trophoblast cells and decidual cells also showed gestational stage-dependent differences. The trajectories of the villous core cells indicated that placental mesenchymal cells were derived from extraembryonic mesoderm (ExE.Meso) 1, whereas placental Hofbauer cells, erythrocytes, and endothelial cells were derived from ExE.Meso2. Comparative analyses of human and macaque placentas uncovered conserved features of placentation across species, and the discrepancies of extravillous trophoblast cells (EVTs) between human and macaque correlated to their differences in invasion patterns and maternal-fetal interactions. Our study provides a groundwork for elucidating the cellular basis of primate placentation.
引用
收藏
页码:806 / +
页数:24
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