This in vitro study evaluated the influence of combined coffee staining and simulated brushing-induced color changes and surface roughness on 3D-printed orthodontic retainers. Specimens measuring 10 x 10 x 0.75 mm(3) were obtained either by conventional vacuum forming or 3D printing at four print angulations (0 degrees, 15 degrees, 30 degrees, and 45 degrees) (n = 10). The prepared specimens were immersed in a coffee beverage and then mechanically brushed using a simulating device. The specimen's color difference (Delta E) and surface roughness (Ra) were quantified using a spectrophotometer and a non-contact profilometer, respectively. The highest and lowest mean Delta E values were recorded for the 3D-printed-45 degrees (4.68 +/- 2.07) and conventional (2.18 +/- 0.87) groups, respectively. The overall mean comparison of Delta E between the conventional and 3D-printed groups was statistically significant (p < 0.01). After simulated brushing, all groups showed a statistically significant increase in the Ra values (p < 0.01). The highest Ra was in the 3D-printed-45 degrees (1.009 +/- 0.13 mu m) and conventional (0.743 +/- 0.12 mu m) groups, respectively. The overall Delta E of 3D-printed orthodontic retainers was not comparable to conventional VFRs. Among the different angulations used to print the retainers, 15 degrees angulations were the most efficient in terms of color changes and surface roughness and were comparable to conventional VFRs.