A photoactivatable and phenylboronic acid-functionalized nanoassembly for combating multidrug-resistant gram-negative bacteria and their biofilms

被引:8
作者
Zhou, Xiaoqing [1 ]
Dong, Lanlan [2 ]
Zhao, Baohua [2 ]
Hu, Guangyun [2 ]
Huang, Can [2 ]
Liu, Tengfei [3 ]
Lu, Yifei [2 ]
Zheng, Mengxue [1 ]
Yu, Yanlan [1 ]
Yang, Zengjun [1 ]
Cheng, Shaowen [4 ]
Xiong, Yan [5 ]
Luo, Gaoxing [2 ]
Qian, Wei [2 ]
Yin, Rui [1 ]
机构
[1] Army Med Univ Third Mil Med Univ, Southwest Hosp, Dept Dermatol, 29 Gaotanyan Rd, Chongqing 400038, Peoples R China
[2] Army Med Univ Third Mil Med Univ, Southwest Hosp, Inst Burn Res, State Key Lab Trauma Burn & Combined Injury, 29 Gaotanyan Rd, Chongqing 400038, Peoples R China
[3] 906 Hosp Joint Logist Support Force PLA, Dept Burn & Plast Sugery, 377 Zhongshan East Rd, Ningbo 315100, Peoples R China
[4] Hainan Med Univ, Dept Wound Repair, Affiliated Hosp 1, 31 Longhua Rd, Haikou 570102, Hainan, Peoples R China
[5] Army Med Univ Third Mil Med Univ, Daping Hosp, Dept Orthopaed, 10 Changjiang Branch Rd, Chongqing 400042, Peoples R China
基金
中国国家自然科学基金;
关键词
Photothermal therapy; Photodynamic therapy; Nitric oxide; Synergistic; Boronic acid; Multidrug-resistant gram-negative bacteria; NITRIC-OXIDE; NANOWORMS; MEMBRANE;
D O I
10.1093/burnst/tkad041
中图分类号
R4 [临床医学];
学科分类号
1002 ; 100602 ;
摘要
Background: Multidrug-resistant (MDR) gram-negative bacteria-related infectious diseases have caused an increase in the public health burden and mortality. Moreover, the formation of biofilms makes these bacteria difficult to control. Therefore, developing novel interventions to combat MDR gram-negative bacteria and their biofilms-related infections are urgently needed. The purpose of this study was to develop a multifunctional nanoassembly (IRNB) based on IR-780 and N, N'-di-sec-butyl-N, N'-dinitroso-1,4-phenylenediamine (BNN6) for synergistic effect on the infected wounds and subcutaneous abscesses caused by gram-negative bacteria. Methods: The characterization and bacteria-targeting ability of IRNB were investigated. The bactericidal efficacy of IRNB against gram-negative bacteria and their biofilms was demonstrated by crystal violet staining assay, plate counting method and live/dead staining in vitro. The antibacterial efficiency of IRNB was examined on a subcutaneous abscess and cutaneous infected wound model in vivo. A cell counting kit-8 assay, Calcein/PI cytotoxicity assay, hemolysis assay and intravenous injection assay were performed to detect the biocompatibility of IRNB in vitro and in vivo. Results: Herein, we successfully developed a multifunctional nanoassembly IRNB based on IR-780 and BNN6 for synergistic photothermal therapy (PTT), photodynamic therapy (PDT) and nitric oxide (NO) effect triggered by an 808 nm laser. This nanoassembly could accumulate specifically at the infected sites of MDR gram-negative bacteria and their biofilms via the covalent coupling effect. Upon irradiation with an 808 nm laser, IRNB was activated and produced both reactive oxygen species (ROS) and hyperthermia. The local hyperthermia could induce NO generation, which further reacted with ROS to generate ONOO-, leading to the enhancement of bactericidal efficacy. Furthermore, NO and ONOO- could disrupt the cell membrane, which converts bacteria to an extremely susceptible state and further enhances the photothermal effect. In this study, IRNB showed a superior photothermal-photodynamic-chemo (NO) synergistic therapeutic effect on the infected wounds and subcutaneous abscesses caused by gram-negative bacteria. This resulted in effective control of associated infections, relief of inflammation, promotion of re-epithelization and collagen deposition, and regulation of angiogenesis during wound healing. Moreover, IRNB exhibited excellent biocompatibility, both in vitro and in vivo. Conclusions: The present research suggests that IRNB can be considered a promising alternative for treating infections caused by MDR gram-negative bacteria and their biofilms.
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页数:27
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