Single-cell N6-methyladenosine-related genes function within the tumor microenvironment to affect the prognosis and treatment sensitivity in patients with gastric cancer

被引：2

作者：

Wang, Zehua ^{[1
]}

Chen, Chen ^{[1
]}

Shu, Jiao ^{[2
]}

Ai, Jiaoyu ^{[3
]}

Liu, Yihan ^{[1
]}

Cao, Haoyue ^{[1
]}

Jia, Yongxu ^{[1
]}

Qin, Yanru ^{[1
]}

机构：

[1] Zhengzhou Univ, Affiliated Hosp 1, Dept Oncol, Zhengzhou 450052, Peoples R China

[2] Zhengzhou Univ, Affiliated Hosp 1, Dept Pathol, Zhengzhou 450052, Peoples R China

[3] Nanchang Univ, Affiliated Hosp 1, Nanchang 330006, Peoples R China

来源：

CANCER CELL INTERNATIONAL | 2024年 / 24卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Gastric cancer; Single-cell analysis; m(6)A; Tumor microenvironment; Prognosis; FIBROBLASTS;

D O I：

10.1186/s12935-024-03227-2

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Background Gastric cancer (GC) ranks fifth for morbidity and third for mortality worldwide. The N-6-methyladenosine (m(6)A) mRNA methylation is crucial in cancer biology and progression. However, the relationship between m(6)A methylation and gastric tumor microenvironment (TME) remains to be elucidated. Methods We combined single-cell and bulk transcriptome analyses to explore the roles of m(6)A-related genes (MRG) in gastric TME. Results Nine TME cell subtypes were identified from 23 samples. Fibroblasts were further grouped into four subclusters according to different cell markers. M(6)A-mediated fibroblasts may guide extensive intracellular communications in the gastric TME. The m(6)A-related genes score (MRGs) was output based on six differentially expressed single-cell m(6)A-related genes (SCMRDEGs), including GHRL, COL4A1, CAV1, GJA1, TIMP1, and IGFBP3. The protein expression level was assessed by immunohistochemistry. We identified the prognostic value of MRGs and constructed a nomogram model to predict GC patients' overall survival. MRGs may affect treatment sensitivity in GC patients. Conclusion Our study visualized the cellular heterogeneity of TME at the single-cell level, revealed the association between m(6)A mRNA modification and intracellular communication, clarified MRGs as an independent risk factor of prognosis, and provided a reference for follow-up treatment.

引用

页数：13

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