Evaluation of expression pattern of cellular miRNAs (let-7b, miR-29a, miR-126, miR-34a, miR-181a-5p) and IL-6, TNF-α, and TGF-β in patients with pseudoexfoliation syndrome

被引：9

作者：

Banasaz, Bahar ^{[1
]}

Zamzam, Razieh ^{[2
]}

Aghadoost, Davood ^{[3
]}

Golabchi, Khodayar ^{[3
]}

Morshedi, Mohammadamin ^{[2
]}

Bayat, Mobina ^{[4
]}

Nahand, Javid Sadri ^{[4
]}

Sheida, Amirhossein ^{[2
]}

Eshraghi, Reza ^{[2
]}

Rahimzadeh, Zoha ^{[2
]}

Mosavi, Seyed Gholamabbas ^{[5
]}

Goleij, Pouya ^{[6
]}

Rezaee, Aryan ^{[7
]}

Mirzaei, Hamed ^{[8
]}

机构：

[1] Babol Univ Med Sci, Internal Med Dept, Babol, Iran

[2] Kashan Univ Med Sci, Sch Med, Kashan, Iran

[3] Kashan Univ Med Sci, Matini Hosp, Dept Ophthalmol, Kashan, Iran

[4] Tabriz Univ Med Sci, Infect & Trop Dis Res Ctr, Tabriz, Iran

[5] Kashan Univ Med Sci, Hlth Fac, Biostat Grp, Kashan, Iran

[6] Sana Inst Higher Educ, Fac Biol, Dept Genet, Sari, Iran

[7] Iran Univ Med Sci, Student Res Comm, Sch Med, Tehran, Iran

[8] Kashan Univ Med Sci, Res Ctr Biochem & Nutr Metab Dis, Inst Basic Sci, Kashan, Iran

来源：

PATHOLOGY RESEARCH AND PRACTICE | 2023年 / 249卷

关键词：

Pseudoexfoliation syndrome; MicroRNAs; Inflammatory cytokines; NECROSIS-FACTOR-ALPHA; HUMAN TRABECULAR MESHWORK; AQUEOUS-HUMOR; OXIDATIVE STRESS; EXTRACELLULAR-MATRIX; DISTINCT CYTOKINE; BINDING-PROTEIN; SERUM-LEVELS; INTERLEUKIN-6; PATHOGENESIS;

D O I：

10.1016/j.prp.2023.154721

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

Pseudoexfoliation syndrome (PEX) is a critical clinical and biological extracellular matrix systemic disorder. Despite the unknown nature of PEX etiopathogenesis, it is proven to be associated with various genes and factors. The present research focused on analyzing the expression of miR and inflammatory cytokines in PEX. Serum and aqueous humor (AH) were collected prior to cataract surgery or trabeculectomy from 99 participants (64 with PEX glaucoma, and 35 controls). Real-time PCR was used for assessing the expression pattern of some miRNAs namely let-7b, miR-29a, miR-126, miR-34a, and miR-181a-5p. ELISA was carried out to explore the transcription of some inflammatory cytokines such as TGF-beta, TNF-alpha, and IL-6. The indication of our results was a significant enhancement in the expression of let-7, miR-34a, and miR-181a-5p in PEX in contrast to the control group. Notwithstanding a significant suppression in miR-29a, and miR-126 expression levels in PEX in contrast to the control group. Analysis of ROC curve revealed that miR-29a and miR-34a are able to act as useful markers in order to discriminate the PEX group from the PEX negative subjects which were determined as the control group. According to the results obtained, the mean levels of TGF-beta, TNF-alpha, and IL-6 upregulated among PEX subjects in contrast to control samples. In conclusion, our findings indicated that the selected cytokines alongside the selected miRNAs could be introduced as a biomarker panel in the diagnosis of PEX.

引用

页数：10