A novel angiotensin-converting enzyme (ACE) inhibitory peptide from tilapia skin: Preparation, identification and its potential antihypertensive mechanism

被引：56

作者：

Dong, Ye ^{[1
]}

Yan, Wen ^{[1
]}

Zhang, Yi-Qi ^{[1
]}

Dai, Zhi-Yuan ^{[1
]}

机构：

[1] Zhejiang Gongshang Univ, Inst Seafood, Key Lab Aquat Prod Proc Zhejiang Prov, Collaborat Innovat Ctr Seafood Deep Proc, Hangzhou 310035, Peoples R China

来源：

FOOD CHEMISTRY | 2024年 / 430卷

基金：

中国国家自然科学基金;

关键词：

Fish skin; Steam explosion; Purification; ACE inhibitory peptides; Molecular docking; BIOACTIVE PEPTIDES; MOLECULAR DOCKING; PURIFICATION; HYDROLYSIS; PROTEINS; COLLAGEN; SAFETY; ASSAY;

D O I：

10.1016/j.foodchem.2023.137074

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

To obtain food-derived peptides with high ACE inhibitory activity, tilapia skin was pretreated with steam explosion prior to enzymatic hydrolysis. The results showed that steam explosion pretreatment improved the hydrolysis efficiency and ACE inhibitory activity of fish skin hydrolysates. A novel ACE inhibitory peptide VGLFPSRSF (1009.17 Da) was obtained from steam-exploded fish skin hydrolysates. VGLFPSRSF had an IC50 value of 61.43 mu M for ACE inhibitory activity, showing a non-competitive binding mode and gastrointestinal enzyme hydrolysis resistance. Molecular docking results showed that VGLFPSRSF interacted with ACE receptor protein through hydrogen bonding and hydrophobic interactions. Based on the results of network pharmacological analysis and molecular docking, VGLFPSRSF might regulate blood pressure through interaction with hypertensive targets such as AKT1, ACE, CD4, REN, and MMP9. Steam-exploded tilapia skin peptides had potential antihypertension activity and might be promising to achieve high-value utilization of fish skin byproducts.

引用

页数：11

共 42 条

[1]

Abdelhedi O, 2018, FOOD CHEM, V239, P453, DOI [10.1016/j.foodchem.2018.01.033, 10.1016/j.foodchem.2017.06.112]

[2] Sequential extraction of gel-forming proteins, collagen and collagen hydrolysate from gutted silver carp (Hypophthalmichthys molitrix), a biorefinery approach [J].