Distinct separation of multiple CTCs using inertial focusing phenomena utilizing single-looped spiral microfluidic lab-on-chip

Looking towards the point-of-care diagnosis purposes, nowadays the most emerging method for separa-tion of Circulating Tumor Cells (CTCs) from normal blood-cells is the inertial focusing phenomena utiliz-ing microfluidic lab-on-chips. Keeping eye on the distinctly separating multiple CTCs from normal blood -cells, here we designed a single-looped spiral microfluidic lab-on-chip based on inertial focusing phe-nomena, which has been more efficient than double-looped and three-looped spirals. The performance of the device has been investigated using large MCF-7 (breast-cancer CTCs), PC3-9 (prostate-cancer CTCs) and normal blood-cells (white blood-cell, red blood-cell) and also larger multiple CTCs like largest MCF-7, PC3-9, A549 (lung-cancer CTCs) cells and normal blood-cells in three-dimensional numerical sim-ulation utilizing COMSOL Multiphysics 5.4 software. In both cases the chip has accomplished efficient separation performance of nearly 100 % at the Reynolds number ranges from 55.9 -65 (throughput 70 -82 ml/h) and 114.74 (throughput of 147 ml/h) respectively in terms of cell separation purity and recovery.(c) 2023 Elsevier Ltd. All rights reserved.