Distinct separation of multiple CTCs using inertial focusing phenomena utilizing single-looped spiral microfluidic lab-on-chip

被引：18

作者：

Pakhira, Writtick ^{[1
]}

Kumar, R. ^{[1
]}

Ibrahimi, Khalid Mohd. ^{[1
]}

机构：

[1] Natl Inst Technol, Dept Elect & Instrumentat Engn, Dimapur 797103, Nagaland, India

来源：

CHEMICAL ENGINEERING SCIENCE | 2023年 / 275卷

关键词：

CTC separation; Inertial focusing; Spiral microfluidic lab -on -chip; Cell recovery; Point-of-care; CIRCULATING TUMOR-CELLS;

D O I：

10.1016/j.ces.2023.118724

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

Looking towards the point-of-care diagnosis purposes, nowadays the most emerging method for separa-tion of Circulating Tumor Cells (CTCs) from normal blood-cells is the inertial focusing phenomena utiliz-ing microfluidic lab-on-chips. Keeping eye on the distinctly separating multiple CTCs from normal blood -cells, here we designed a single-looped spiral microfluidic lab-on-chip based on inertial focusing phe-nomena, which has been more efficient than double-looped and three-looped spirals. The performance of the device has been investigated using large MCF-7 (breast-cancer CTCs), PC3-9 (prostate-cancer CTCs) and normal blood-cells (white blood-cell, red blood-cell) and also larger multiple CTCs like largest MCF-7, PC3-9, A549 (lung-cancer CTCs) cells and normal blood-cells in three-dimensional numerical sim-ulation utilizing COMSOL Multiphysics 5.4 software. In both cases the chip has accomplished efficient separation performance of nearly 100 % at the Reynolds number ranges from 55.9 -65 (throughput 70 -82 ml/h) and 114.74 (throughput of 147 ml/h) respectively in terms of cell separation purity and recovery.(c) 2023 Elsevier Ltd. All rights reserved.

引用

页数：19

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