Prevalence of Clostridioides difficile in dogs (Canis familiaris) with gastrointestinal disorders in Rio de Janeiro

被引：4

作者：

Leite, Suzana ^{[1
,2
]}

Cotias, Carlos ^{[6
]}

Rainha, Kelly C. ^{[2
]}

Santos, Mayara Gil ^{[2
]}

Penna, Bruno ^{[5
]}

Moraes, Renata F. F. ^{[4
]}

Harmanus, Celine

Smits, Wiep Klaas ^{[3
]}

Ferreira, Eliane de Oliveira ^{[1
,7
]}

机构：

[1] Univ Fed Rio Janeiro, Departmento Microbiol Med, Inst Microbiol Paulo Goes IMPG, Rio De Janeiro, Brazil

[2] Univ Santa Ursula, Rio De Janeiro, Brazil

[3] Leiden Univ, Dept Med Microbiol, Med Ctr, Leiden, Netherlands

[4] Univ Vassouras, Vassouras, Brazil

[5] Univ Fed Fluminense UFF, Departmento Microbiol & Parasitol, Inst Biomed, Niteroi, Brazil

[6] Vet Diag Ctr, H&Diagnost, Rio De Janeiro, Brazil

[7] IMPG, Dept Microbiol Med, CCS, Lab Biol Anaerobios Cep, Av Carlos Chagas Filho 373,Bloco I-2,Sala 06, BR-20720350 Rio De Janeiro, Brazil

来源：

ANAEROBE | 2023年 / 83卷

关键词：

Bacterial epidemiology; Clostridioides difficile; Canis familiaris; Gastrointestinal disorders; ENZYME IMMUNOASSAYS; CATS DIAGNOSIS; PCR RIBOTYPES; PERFRINGENS; STRAINS; INFECTION; CARRIAGE; TOXIN; RESISTANCE; DIARRHEA;

D O I：

10.1016/j.anaerobe.2023.102765

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Clostridioides difficile infections (CDI) have a high morbidity and mortality rate and have always been considered a nosocomial disease. Nonetheless, the number of cases of community-acquired CDI is increasing, and new evidence suggests additional C. difficile reservoirs exist. Pathogenic C. difficile strains have been found in livestock, domestic animals, and meat, so a zoonotic transmission has been proposed. Objective: The goal of this study was to isolate C. difficile strains in dogs at a veterinary clinic in Rio de Janeiro, Brazil, and characterize clinical and pathological findings associated with lower gastrointestinal tract disorders. Methods: Fifty stool samples and biopsy fragments from dogs were obtained and cultured in the CDBA selective medium. All suggestive C. difficile colonies were confirmed by MALDI-TOF MS and PCR (tpi gene). Vancomycin, metronidazole, moxifloxacin, erythromycin, and rifampicin were tested for antibiotic susceptibility. Biofilm, motility assays, and a PCR for the toxins (tcdA, tcdB, and cdtB), as well as ribotyping, were also performed. Results: Blood samples and colonic biopsy fragments were examined in C. difficile positive dogs. Ten animals (20%) tested positive for C. difficile by using stool samples, but not from biopsy fragments. Most C. difficile strains were toxigenic: six were A+B+ belonging to RT106; two were A+B+ belonging to RT014/020; and two were AB- belonging to RT010. All strains were biofilm producers. In the motility test, 40% of strains were as motile as the positive control, CD630 (RT012). In the disc diffusion test, two strains (RT010) were resistant to erythromycin and metronidazole; and another to metronidazole (RT014/020). In terms of C. difficile clinicopathological correlations, no statistically significant morphological changes, such as pseudomembranous and "volcano" lesions, were observed. Regarding hematological data, dogs positive for C. difficile had leucopenia (p = 0.02) and lymphopenia (p = 0.03). There was a significant correlation between senility and the presence of C. difficile in the dogs studied (p = 0,02). Conclusions: Although C. difficile has not been linked to canine diarrheal disorders, it appears to be more common in dogs with intestinal dysfunctions. The isolation of ribotypes frequently involved in human CDI outbreaks around the world supports the theory of C. difficile zoonotic transmission.

引用

页数：10