Assessing locomotory rate in response to food for the identification of neuronal and muscular defects in C. elegans

被引：2

作者：

Petratou, Dionysia ^{[1
,2
]}

Fragkiadaki, Persefoni ^{[3
]}

Lionaki, Eirini ^{[1
]}

Tavernarakis, Nektarios ^{[1
,2
]}

机构：

[1] Fdn Res & Technol, Inst Mol Biol & Biotechnol, Iraklion 70013, Crete, Greece

[2] Univ Crete, Med Sch, Dept Basic Sci, Iraklion 71003, Crete, Greece

[3] Univ Crete, Med Sch, Dept Toxicol, Iraklion 71003, Crete, Greece

来源：

STAR PROTOCOLS | 2024年 / 5卷 / 01期

基金：

欧洲研究理事会; 美国国家卫生研究院; 欧盟地平线“2020”;

关键词：

Behavior; Genetics; Microscopy; Model Organisms; Signal Transduction;

D O I：

10.1016/j.xpro.2023.102801

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

C. elegans is a bacteria-eating soil-dwelling nematode. Typical cultivation of lab-oratory-reared populations occurs on bacteria-covered solid media, where they move along with sinusoidal undulations. Nematodes decelerate when they encounter food. Dopaminergic and serotonergic neurotransmission regulate this behavior. Here, we describe the procedure for determining food-dependent locomotion rate of fed and fasting nematodes. We detail steps for assay plate preparation, C. elegans synchronization, and assessment of locomotion. The be-haviors we describe provide information regarding the animal's physiological neuronal and muscular function. For complete details on the use and execution of this protocol, please refer to Petratou et al. (2023)1 and Sawin et al. (2000).2

引用

页数：16

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