Underrepresentation of activating KIR gene expression in single-cell RNA-seq data is due to KIR gene misassignment

被引：0

作者：

Alves, Eric ^{[1
,2
]}

Chopra, Abha ^{[3
]}

Ram, Ramesh ^{[3
]}

Currenti, Jennifer ^{[2
,4
]}

Kalams, Spyros A. ^{[5
]}

Mallal, Simon A. ^{[3
,5
]}

Phillips, Elizabeth J. ^{[3
,5
]}

Gaudieri, Silvana ^{[1
,3
,5
,6
]}

机构：

[1] Univ Western Australia, Sch Human Sci, Crawley, WA, Australia

[2] QEII Med Ctr, Harry Perkins Inst Med Res, Nedlands, WA, Australia

[3] Murdoch Univ, Inst Immunol & Infect Dis, Murdoch, WA, Australia

[4] Curtin Univ, Sch Med, Bentley, WA, Australia

[5] Vanderbilt Univ, Med Ctr, Dept Med, Nashville, TN USA

[6] Univ Western Australia, Sch Human Sci, Perth, WA 6009, Australia

来源：

EUROPEAN JOURNAL OF IMMUNOLOGY | 2024年 / 54卷 / 01期

基金：

美国国家卫生研究院; 英国医学研究理事会;

关键词：

HLA;

D O I：

10.1002/eji.202350590

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Standard single-cell RNA-sequencing alignment pipelines exhibit a propensity for misassigning killer immunoglobulin-like receptor (KIR) transcripts, thereby giving rise to inaccuracies in quantifying KIR expression. Alves et al. elucidated that these default workflows frequently misclassify activating KIR transcripts as inhibitory KIR expression, resulting in a skewed representation of the KIR repertoire.

引用

页数：4

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