A specific high-performance thin-layer chromatography method validated for estimation of mometasone furoate and olopatadine hydrochloride

High-performance thin-layer chromatography has the capacity to run many samples simultaneously and reduced solvent usage, It offers a number of advantages that can save both time and money. The simultaneous quantification of mometasone furoate and olopatadine hydrochloride in a combined dose has been developed and validated using a specific, precise, accurate, and economical high-performance thin-layer chromatography technique. Methanol: Water (8: 2, v/v) was used as the mobile phase during the separation, which took place on a stationary phase of pre-coated silica gel aluminum plate 60 F-254. At 248 nm, separated components were densitometrically measured. The method was validated for Linearity and range, precision, reproducibility, specificity, accuracy, the limit of detection, and the limit of quantification as per the International Council for Harmonization Q2(R1) guideline. mometasone furoate and olopatadine hydrochloride regression coefficients (R2) were found to be 0.9995 and 0.9991, accordingly. Mometasone furoate and olopatadine hydrochloride percentage recoveries were measured to be 98.99 +/- 0.39 and 99.15 +/- 0.95, respectively. The analytical findings demonstrate that the proposed approach could be used to quantify mometasone furoate and olopatadine HCl simultaneously in a combined dose.