The potential role of serum extracellular vesicle derived small RNAs in AML research as non-invasive biomarker

被引:7
作者
Li, Lin [1 ]
Mussack, Veronika [2 ]
Goergens, Andre [3 ,4 ]
Pepeldjiyska, Elena [1 ]
Hartz, Anne Sophie [1 ]
Aslan, Hazal [1 ]
Rackl, Elias [1 ]
Rank, Andreas [5 ]
Schmohl, Joerg [6 ]
El Andaloussi, Samir [3 ]
Pfaffl, Michael W. [2 ]
Schmetzer, Helga [1 ]
机构
[1] Univ Hosp Munich, Immune Modulat Med Dept 3, Marchioninistr 15, D-81377 Munich, Germany
[2] Tech Univ Munich, TUM Sch Life Sci Weihenstephan, Dept Anim Physiol & Immunol, Freising Weihenstephan, Germany
[3] Karolinska Inst, Dept Lab Med, Div Biomol & Cellular Med, Stockholm, Sweden
[4] Univ Duisburg Essen, Univ Hosp Essen, Inst Transfus Med, Essen, Germany
[5] Univ Hosp Augsburg, Dept Hematol & Oncol, Augsburg, Germany
[6] Hosp Stuttgart, Dept Hematol & Oncol, Stuttgart, Germany
来源
NANOSCALE ADVANCES | 2023年 / 5卷 / 06期
关键词
ACUTE MYELOID-LEUKEMIA; PROGNOSTIC BIOMARKER; MICRORNA; EXPRESSION; EXOSOMES; CELLS; SURVIVAL;
D O I
10.1039/d2na00959e
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Background: Extracellular vesicles (EV) are cell-derived vesicles released by all cells in health and disease. Accordingly, EVs are also released by cells in acute myeloid leukemia (AML), a hematologic malignancy characterized by uncontrolled growth of immature myeloid cells, and these EVs likely carry markers and molecular cargo reflecting the malignant transformation occurring in diseased cells. Monitoring antileukemic or proleukemic processes during disease development and treatment is essential. Therefore, EVs and EV-derived microRNA (miRNA) from AML samples were explored as biomarkers to distinguish disease-related patterns ex vivo or in vivo. Methodology: EVs were purified from serum of healthy (H) volunteers and AML patients by immunoaffinity. EV surface protein profiles were analyzed by multiplex bead-based flow cytometry (MBFCM) and total RNA was isolated from EVs prior to miRNA profiling via small RNA sequencing. Results: MBFCM revealed different surface protein patterns in H versus AML EVs. miRNA analysis showed individual as well as highly dysregulated patterns in H and AML samples. Conclusions: In this study, we provide a proof-of-concept for the discriminative potential of EV derived miRNA profiles as biomarkers in H versus AML samples.
引用
收藏
页码:1691 / 1705
页数:15
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