Identification of Two Novel Linear B Cell Epitopes on the CD2v Protein of African Swine Fever Virus Using Monoclonal Antibodies

被引:7
作者
Jiang, Wenting [1 ,2 ]
Jiang, Dawei [1 ,2 ,3 ]
Li, Lu [1 ,2 ]
Wang, Jiabin [1 ,2 ]
Wang, Panpan [1 ,2 ]
Shi, Xuejian [1 ,2 ]
Zhao, Qi [1 ,2 ]
Liu, Boyuan [1 ,2 ]
Ji, Pengchao [1 ,2 ,4 ]
Zhang, Gaiping [1 ,2 ,3 ,4 ]
机构
[1] Henan Agr Univ, Coll Vet Med, Zhengzhou 450046, Peoples R China
[2] Int Joint Res Ctr Natl Anim Immunol, Zhengzhou 450046, Peoples R China
[3] Longhu Lab, Zhengzhou 450046, Peoples R China
[4] Henan Engn Lab Anim Biol Prod, Zhengzhou 450046, Peoples R China
来源
VIRUSES-BASEL | 2023年 / 15卷 / 01期
基金
中国国家自然科学基金;
关键词
African swine fever virus; CD2v; identification; B cell epitope; monoclonal antibody; CD2-LIKE GENE; DELETION; GEORGIA; CHALLENGE; SAFETY; ASSAY;
D O I
10.3390/v15010131
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
African swine fever virus (ASFV) is a highly infectious viral pathogen that endangers the global pig industry, and no effective vaccine is available thus far. The CD2v protein is a glycoprotein on the outer envelope of ASFV, which mediates the transmission of the virus in the blood and recognition of the virus serotype, playing an important role in ASFV vaccine development and disease prevention. Here, we generated two specific monoclonal antibodies (mAbs), 6C11 and 8F12 (subtype IgG1/kappa-type), against the ASFV CD2v extracellular domain (CD2v-ex, GenBank: MK128995.1, 1-588 bp) and characterized their specificity. Peptide scanning technology was used to identify the epitopes recognized by mAbs 6C11 and 8F12. As a result, two novel B cell epitopes, (38)DINGVSWN(45) and (134)GTNTNIY(140), were defined. Amino acid sequence alignment showed that the defined epitopes were conserved in all referenced ASFV strains from various regions of China including the highly pathogenic, epidemic strain, Georgia2007/1 (NC_044959.2), with the same noted substitutions compared to the four foreign ASFV wild-type strains. This study provides important reference values for the design and development of an ASFV vaccine and useful biological materials for the functional study of the CD2v protein by deletion analysis.
引用
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页数:13
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