Estimation of postmortem interval using top-down HPLC-MS analysis of peptide fragments in vitreous humour: A pilot study

被引：4

作者：

Boroumand, Mozhgan ^{[1
]}

Grassi, Vincenzo M. ^{[2
,3
]}

Castagnola, Flaminia ^{[2
]}

De-Giorgio, Fabio ^{[2
,3
,9
]}

d'Aloja, Ernesto ^{[4
]}

Vetrugno, Giuseppe ^{[2
,3
]}

Pascali, Vincenzo L. ^{[2
,3
,9
]}

Vincenzoni, Federica ^{[3
,5
]}

Iavarone, Federica ^{[3
,5
]}

Faa, Gavino ^{[6
,7
]}

Castagnola, Massimo ^{[8
]}

机构：

[1] NIA, Translat Gerontol Branch, NIH, Baltimore, MD 21224 USA

[2] Univ Cattolica Sacro Cuore, Dept Healthcare Surveillance & Bioeth, Sect Legal Med, Rome, Italy

[3] Fdn Policlin Univ IRCCS A Gemelli, Rome, Italy

[4] Univ Cagliari, Dept Med Sci & Publ Hlth, Sect Legal Med, Cagliari, Italy

[5] Univ Cattolica Sacro Cuore, Fac Med & Chirurg, Dipartimento Sci Biotecnol Base Clin Intensivol &, Rome, Italy

[6] Univ Cagliari, Dipartimento Sci Med & Sanita Pubbl, Sez Anat Patol, Cagliari, Italy

[7] Temple Univ, Philadelphia, PA USA

[8] IRCCS Fdn Santa Lucia, Ctr Europeo Ric Cervello, Rome, Italy

[9] Univ Cattolica Sacro Cuore, Dept Healthcare Surveillance & Bioeth, Sect Legal Med, Rome, Italy

来源：

INTERNATIONAL JOURNAL OF MASS SPECTROMETRY | 2023年 / 483卷

关键词：

Postmortem interval; Time of death; Top-down HPLC-MS analysis; Peptide fragments; Vitreous humour; Forensic autopsy; Postmortem investigation; MASS-SPECTROMETRY; PROTEOMICS; CHEMISTRY; DEGRADATION; DECAY; TIME; EYE;

D O I：

10.1016/j.ijms.2022.116952

中图分类号：

O64 [物理化学（理论化学）、化学物理学]; O56 [分子物理学、原子物理学];

学科分类号：

070203 ; 070304 ; 081704 ; 1406 ;

摘要：

This study reports the detection of a set of 35 peptide fragments and 7 intact proteins in the vitreous humour using a top-down proteomic platform based on high-resolution HPLC-MS and MS/MS analysis. The concentrations of thymosin b4 (R = 0.932) and two peptide fragments (i.e., vimentin fragment (Fr.) 443-465 (R = 0.998) and polyubiquitin Fr. 1-73 (R = 0.968)) were observed to have very strong linear correlations with postmortem intervals. Data are available via ProteomeXchange with identifier PXD037095. These preliminary results suggest that some biochemical molecular events are linearly related to the postmortem interval and that the concentrations of these peptides and fragments can be clinically useful in establishing the time of death if measured within the first 160 h postmortem. (c) 2022 Elsevier B.V. All rights reserved.

引用

页数：8

共 43 条

[21] Digging Deeper for the Eye Proteome in Vitreous Substructures: A High-Resolution Proteome Map of the Normal Human Vitreous Base [J].

Mohanty, Varshasnata ;

Pinto, Sneha M. ;

Subbannayya, Yashwanth ;

Najar, Mohd. Altaf ;

Murthy, Kalpana Babu ;

Prasad, Thottethodi Subrahmanya Keshava ;

Murthy, Krishna R. .

OMICS-A JOURNAL OF INTEGRATIVE BIOLOGY, 2020, 24 (06) :379-389

[22] Dominant cataract formation in association with a vimentin assembly disrupting mutation [J].

Mueller, Martin ;

Bhattacharya, Shomi S. ;

Moore, Tony ;

Prescott, Quincy ;

Wedig, Tatjana ;

Herrmann, Harald ;

Magin, Thomas M. .

HUMAN MOLECULAR GENETICS, 2009, 18 (06) :1052-1057

[23] Repeatability and reproducibility of post-mortem central corneal thickness measurements using a portable optical coherence tomography system in humans: a prospective multicenter study [J].

Napoli, Pietro Emanuele ;

Nioi, Matteo ;

Gabiati, Letizia ;

Laurenzo, Michela ;

De-Giorgio, Fabio ;

Scorcia, Vincenzo ;

Grassi, Simone ;

D'Aloja, Ernesto ;

Fossarello, Maurizio .

SCIENTIFIC REPORTS, 2020, 10 (01)

[24] Sorting of non-glycosylated human procathepsin S in mammalian cells [J].

Nissler, K ;

Kreusch, S ;

Rommerskirch, W ;

Strubel, W ;

Weber, E ;

Wiederanders, B .

BIOLOGICAL CHEMISTRY, 1998, 379 (02) :219-224

[25] Mass spectrometry-based proteomics turns quantitative [J].

Ong, SE ;

Mann, M .

NATURE CHEMICAL BIOLOGY, 2005, 1 (05) :252-262

[26] Postmortem chemistry update part II [J].

Palmiere, Cristian ;

Mangin, Patrice .

INTERNATIONAL JOURNAL OF LEGAL MEDICINE, 2012, 126 (02) :199-215

[27]

Palmiere C, 2012, INT J LEGAL MED, V126, P187, DOI 10.1007/s00414-011-0625-y

[28] Forensic proteomics [J].

Parker, Glendon J. ;

McKiernan, Heather E. ;

Legg, Kevin M. ;

Goecker, Zachary C. .

FORENSIC SCIENCE INTERNATIONAL-GENETICS, 2021, 54

[29] The PRIDE database resources in 2022: a hub for mass spectrometry-based proteomics evidences [J].

Perez-Riverol, Yasset ;

Bai, Jingwen ;

Bandla, Chakradhar ;

Garcia-Seisdedos, David ;

Hewapathirana, Suresh ;

Kamatchinathan, Selvakumar ;

Kundu, Deepti J. ;

Prakash, Ananth ;

Frericks-Zipper, Anika ;

Eisenacher, Martin ;

Walzer, Mathias ;

Wang, Shengbo ;

Brazma, Alvis ;

Vizcaino, Juan Antonio .

NUCLEIC ACIDS RESEARCH, 2022, 50 (D1) :D543-D552

[30] Vitreous humor endogenous compounds analysis for post-mortem forensic investigation [J].

Pigaiani, Nicola ;

Bertaso, Anna ;

De Palo, Elio Franco ;

Bortolotti, Federica ;

Tagliaro, Franco .

FORENSIC SCIENCE INTERNATIONAL, 2020, 310

← 1 2 3 4 5 →