Estimation of postmortem interval using top-down HPLC-MS analysis of peptide fragments in vitreous humour: A pilot study

被引：3
作者：
Boroumand, Mozhgan [1 ]
Grassi, Vincenzo M. [2 ,3 ]
Castagnola, Flaminia [2 ]
De-Giorgio, Fabio [2 ,3 ,9 ]
d'Aloja, Ernesto [4 ]
Vetrugno, Giuseppe [2 ,3 ]
Pascali, Vincenzo L. [2 ,3 ,9 ]
Vincenzoni, Federica [3 ,5 ]
Iavarone, Federica [3 ,5 ]
Faa, Gavino [6 ,7 ]
Castagnola, Massimo [8 ]
机构：
[1] NIA, Translat Gerontol Branch, NIH, Baltimore, MD 21224 USA
[2] Univ Cattolica Sacro Cuore, Dept Healthcare Surveillance & Bioeth, Sect Legal Med, Rome, Italy
[3] Fdn Policlin Univ IRCCS A Gemelli, Rome, Italy
[4] Univ Cagliari, Dept Med Sci & Publ Hlth, Sect Legal Med, Cagliari, Italy
[5] Univ Cattolica Sacro Cuore, Fac Med & Chirurg, Dipartimento Sci Biotecnol Base Clin Intensivol &, Rome, Italy
[6] Univ Cagliari, Dipartimento Sci Med & Sanita Pubbl, Sez Anat Patol, Cagliari, Italy
[7] Temple Univ, Philadelphia, PA USA
[8] IRCCS Fdn Santa Lucia, Ctr Europeo Ric Cervello, Rome, Italy
[9] Univ Cattolica Sacro Cuore, Dept Healthcare Surveillance & Bioeth, Sect Legal Med, Rome, Italy
来源：
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY | 2023年 / 483卷
关键词：
Postmortem interval; Time of death; Top-down HPLC-MS analysis; Peptide fragments; Vitreous humour; Forensic autopsy; Postmortem investigation; MASS-SPECTROMETRY; PROTEOMICS; CHEMISTRY; DEGRADATION; DECAY; TIME; EYE;
D O I：
10.1016/j.ijms.2022.116952
中图分类号：
O64 [物理化学（理论化学）、化学物理学]; O56 [分子物理学、原子物理学];
学科分类号：
070203 ; 070304 ; 081704 ; 1406 ;
摘要：
This study reports the detection of a set of 35 peptide fragments and 7 intact proteins in the vitreous humour using a top-down proteomic platform based on high-resolution HPLC-MS and MS/MS analysis. The concentrations of thymosin b4 (R = 0.932) and two peptide fragments (i.e., vimentin fragment (Fr.) 443-465 (R = 0.998) and polyubiquitin Fr. 1-73 (R = 0.968)) were observed to have very strong linear correlations with postmortem intervals. Data are available via ProteomeXchange with identifier PXD037095. These preliminary results suggest that some biochemical molecular events are linearly related to the postmortem interval and that the concentrations of these peptides and fragments can be clinically useful in establishing the time of death if measured within the first 160 h postmortem. (c) 2022 Elsevier B.V. All rights reserved.
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页数：8
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