How to educate across differences in the 21st century? Based on this question, the present study focuses on a diversity of subjects that have been gaining greater visibility in public spaces and in educational processes amid the dynamics of inclusion. The issues related to them are multiple, mitigated mainly by their singularities, which reveal exclusions, injustices, inequalities and conflicts. It is at school that bodies encounter difference or, it is there that pure difference emerges in the face of the norm of cultural standardization. Bearing in mind the strength of this problem, the notes that follow aim to engage with some theoretical conceptions of difference and diversity, promoting generating effects that allow dialogue, so that this theme can be worked on in everyday school life in an affirmative way, as well as to the challenges that difference brings to teacher training. For this purpose, writing establishes connections between the theory of knowledge with cultural studies, neurosciences, philosophies of difference and studies of the body. The problematic field of the argument moves between the concepts of difference and inclusion with the concreteness of the school floor, making the term difference a lever of possibilities, ruptures, questions and actions, which affirm education from the perspective of inclusion.