Secured delivery of basic fibroblast growth factor using human serum albumin-based protein nanoparticles for enhanced wound healing and regeneration

被引：19

作者：

Son, Boram ^{[1
]}

Kim, Minju ^{[2
]}

Won, Hyosub ^{[1
]}

Jung, Ara ^{[3
,4
]}

Kim, Jihyun ^{[1
]}

Koo, Yonghoe ^{[2
]}

Lee, Na Kyeong ^{[5
]}

Baek, Seung-Ho ^{[6
]}

Han, Uiyoung ^{[7
]}

Park, Chun Gwon ^{[8
]}

Shin, Heungsoo ^{[1
]}

Gweon, Bomi ^{[3
]}

Joo, Jinmyoung ^{[2
,9
,10
]}

Park, Hee Ho ^{[1
,11
]}

机构：

[1] Hanyang Univ, Dept Bioengn, Seoul, South Korea

[2] Ulsan Natl Inst Sci & Technol UNIST, Dept Biomed Engn, Ulsan, South Korea

[3] Sejong Univ, Dept Mech Engn, Seoul, South Korea

[4] Catholic Univ Korea, Coll Med, Dept Biomed & Hlth Sci, Seoul, South Korea

[5] Sungkyunkwan Univ SKKU, Dept Intelligent Precis Healthcare Convergence, Suwon, South Korea

[6] Korea Res Inst Chem Technol KRICT, Ctr Biobased Chem, Ulsan, South Korea

[7] Stanford Univ, Sch Med, Dept Ophthalmol, Stanford, CA USA

[8] Sungkyunkwan Univ SKKU, SKKU Inst Convergence, Dept Biomed Engn, Suwon, South Korea

[9] Univ Calif San Diego, Mat Res Sci & Engn Ctr, La Jolla, CA 92093 USA

[10] Inst Basic Sci IBS, Ctr Genom Integr, Ulsan, South Korea

[11] Hanyang Univ, Res Inst Convergence Basic Sci, Seoul, South Korea

来源：

JOURNAL OF NANOBIOTECHNOLOGY | 2023年 / 21卷 / 01期

基金：

新加坡国家研究基金会;

关键词：

Basic fibroblast growth factor (bFGF); Human serum albumin (HSA); Protein nanoparticle; Wound healing; Tissue regeneration; DRUG-RELEASE; ELECTROSTATIC INTERACTIONS; DIFFERENTIATION; PROLIFERATION; MIGRATION; THERAPY; ANGIOGENESIS; POLYMER; BFGF;

D O I：

10.1186/s12951-023-02053-4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Background Basic fibroblast growth factor (bFGF) is one of the critical components accelerating angiogenesis and tissue regeneration by promoting the migration of dermal fibroblasts and endothelial cells associated with matrix formation and remodeling in wound healing process. However, clinical applications of bFGF are substantially limited by its unstable nature due to rapid decomposition under physiological microenvironment. Results In this study, we present the bFGF-loaded human serum albumin nanoparticles (HSA-bFGF NPs) as a means of enhanced stability and sustained release platform during tissue regeneration. Spherical shape of the HSA-bFGF NPs with uniform size distribution (polydispersity index < 0.2) is obtained via a simple desolvation and crosslinking process. The HSA-bFGF NPs securely load and release the intact soluble bFGF proteins, thereby significantly enhancing the proliferation and migration activity of human dermal fibroblasts. Myofibroblast-related genes and proteins were also significantly down-regulated, indicating decrease in risk of scar formation. Furthermore, wound healing is accelerated while achieving a highly organized extracellular matrix and enhanced angiogenesis in vivo. Conclusion Consequently, the HSA-bFGF NPs are suggested not only as a delivery vehicle but also as a protein stabilizer for effective wound healing and tissue regeneration.

引用

页数：18