Near-infrared co-illumination of fluorescent proteins reduces photobleaching and phototoxicity

被引：10

作者：

Ludvikova, Lucie ^{[1
]}

Simon, Emma ^{[1
]}

Deygas, Mathieu ^{[2
,3
]}

Panier, Thomas ^{[4
]}

Plamont, Marie-Aude ^{[1
]}

Ollion, Jean ^{[5
]}

Tebo, Alison ^{[1
]}

Piel, Matthieu ^{[2
,3
]}

Jullien, Ludovic ^{[1
]}

Robert, Lydia ^{[4
,6
]}

Le Saux, Thomas ^{[1
]}

Espagne, Agathe ^{[1
]}

机构：

[1] Sorbonne Univ, PSL Univ, CNRS, Ecole Normale Super,Dept Chim,PASTEUR, Paris, France

[2] Paris Sci & Lettres PSL Res Univ, Inst Curie, CNRS, Paris, France

[3] PSL Res Univ, Inst Pierre Gilles Gennes, Paris, France

[4] Sorbonne Univ, Inst Biol Paris Seine IBPS, CNRS, Lab Jean Perrin LJP, Paris, France

[5] SABILab, Die, France

[6] Univ Paris Saclay, Micalis Inst, INRAE, AgroParisTech, Jouy En Josas, France

来源：

NATURE BIOTECHNOLOGY | 2024年 / 42卷 / 06期

关键词：

STATE; TIME; MICROSCOPY; MECHANISM; TRACKING; GFP;

D O I：

10.1038/s41587-023-01893-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Here we present a method to reduce the photobleaching of fluorescent proteins and the associated phototoxicity. It exploits a photophysical process known as reverse intersystem crossing, which we induce by near-infrared co-illumination during fluorophore excitation. This dual illumination method reduces photobleaching effects 1.5-9.2-fold, can be easily implemented on commercial microscopes and is effective in eukaryotic and prokaryotic cells with a wide range of fluorescent proteins. A dual illumination method reduces photobleaching for green and yellow fluorescent proteins.

引用

页码：872 / +

页数：12

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