PV interneurons evoke astrocytic Ca2+ responses in awake mice, which contributes to neurovascular coupling

被引:8
作者
Krogsgaard, Aske [1 ]
Sperling, Leonora [1 ]
Dahlqvist, Matilda [1 ]
Thomsen, Kirsten [1 ]
Vydmantaite, Gabriele [1 ,2 ]
Li, Fangyuan [1 ]
Thunemann, Martin [3 ]
Lauritzen, Martin [1 ,4 ]
Lind, Barbara Lykke [1 ]
机构
[1] Univ Copenhagen, Fac Hlth Sci, Dept Neurosci, DK-2200 Copenhagen, Denmark
[2] Lithuanian Univ Hlth Sci, Inst Pharmaceut Technol, Med Acad, Fac Pharm,Lab Pharmaceut Sci, LT-50162 Kaunas, Lithuania
[3] Boston Univ, Dept Biomed Engn, Boston, MA 02215 USA
[4] Rigshosp, Dept Clin Neurophysiol, DK-2600 Glostrup, Denmark
关键词
astrocytes; brain states; inhibitory interneurons; neurovascular coupling; noradrenaline; BLOOD-FLOW; CALCIUM TRANSIENTS; ANESTHESIA; PERICYTES; ENDFEET; NEURONS; SIGNALS;
D O I
10.1002/glia.24370
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Neurovascular coupling (NVC) modulates cerebral blood flow to match increased metabolic demand during neuronal excitation. Activation of inhibitory interneurons also increase blood flow, but the basis for NVC caused by interneurons is unclear. While astrocyte Ca2+ levels rise with excitatory neural transmission, much less is known with regards to astrocytic sensitivity to inhibitory neurotransmission. We performed two-photon microscopy in awake mice to examine the correlation between astrocytic Ca2+ and NVC, evoked by activation of either all (VGAT(IN)) or only parvalbumin-positive GABAergic interneurons (PVIN). Optogenetic stimulation of VGAT(IN) and PVIN in the somatosensory cortex triggered astrocytic Ca2+ increases that were abolished by anesthesia. In awake mice, PVIN evoked astrocytic Ca2+ responses with a short latency that preceded NVC, whereas VGAT(IN) evoked Ca2+ increases that were delayed relative to the NVC response. The early onset of PVIN evoked astrocytic Ca2+ increases depended on noradrenaline release from locus coeruleus as did the subsequent NVC response. Though the relationship between interneuron activity and astrocytic Ca2+ responses is complex, we suggest that the rapid astrocyte Ca2+ responses to increased PVIN activity shaped the NVC. Our results underline that interneuron and astrocyte-dependent mechanisms should be studied in awake mice.
引用
收藏
页码:1830 / 1846
页数:17
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