Grape seed extract proanthocyanidin antagonizes aristolochic acid I-induced liver injury in rats by activating PI3K-AKT pathway

被引：0
作者：
Wang, Shuang [1 ]
Liu, Zhihui [1 ]
Wang, Yao [1 ]
Shi, Bendong [1 ]
Jin, Yinzhu [1 ]
Wang, Yu [1 ]
Jiang, Xiaowen [1 ]
Song, Mingxin [1 ]
Yu, Wenhui [1 ,2 ,3 ]
机构：
[1] Northeast Agr Univ, Coll Vet Med, Harbin 150038, Peoples R China
[2] Heilongjiang Prov Key Lab Anim Dis Prevent & Cont, Harbin, Peoples R China
[3] Northeast Agr Univ, Inst Tradit Chinese Vet Med, Harbin, Peoples R China
来源：
TOXICOLOGY MECHANISMS AND METHODS | 2023年 / 33卷 / 02期
基金：
中国国家自然科学基金;
关键词：
Aristolochic acid; liver; toxicity; oxidative stress; grape seed proanthocyanidin extract; APOPTOSIS; AUTOPHAGY; BIOSYNTHESIS; CANCER;
D O I：
10.1080/15376516.2022.2103479
中图分类号：
R99 [毒物学（毒理学）];
学科分类号：
100405 ;
摘要：
Aristolochic acid is internationally recognized as a carcinogen. It has been shown that the main toxic mechanism of aristolochic acid on the liver and kidney is the induction of ROS-induced oxidative stress damage. To investigate whether proanthocyanidins (GSPE), a natural antioxidant product from grape seed extract, could antagonize AA-I-induced liver injury. Thirty-two SD rats were selected and divided into aristolochic acid exposure group (AA-I), normal control group, GSPE group and GSPE intervention group. The protective effects of GSPE on AA-I liver injury were evaluated by examining the body weight, liver index, liver function and liver pathological sections of rats. The results of body weight, liver index, liver function and liver pathological sections of rats showed that GSPE had antagonistic effects on AA-I-induced liver injury. antioxidant enzyme activity in the GSPE intervention group was significantly higher than that in the aristolochic acid group, apoptotic cells were significantly lower than that in the aristolochic acid group, protein and mRNA expression of PI3K-AKT and BCL-2 were significantly higher than that in the aristolochic acid group, BAX, The protein and mRNA expression of BAX, CASPAES-3, CASPAES-9 were significantly lower than those of the aristolochic acid group. GSPE can antagonize aristolochic acid-induced hepatotoxicity, and its mechanism of action is to antagonize aristolochic acid I-induced liver injury by inhibiting PI3K-AKT pathway-mediated hepatocyte apoptosis.
引用
收藏
页码：131 / 140
页数：10
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