Purification and Activity of the Second Recombinant Enzyme for Biodegrading Linearized Microcystins by Sphingopyxis sp. USTB-05

被引：2

作者：

Teng, Junhui ^{[1
]}

Song, Meijie ^{[1
]}

Xu, Qianqian ^{[1
]}

Zou, Qianwen ^{[1
]}

Zhang, Haiyang ^{[1
]}

Yin, Chunhua ^{[1
]}

Liu, Xiaolu ^{[1
]}

Liu, Yang ^{[1
]}

Yan, Hai ^{[1
]}

机构：

[1] Univ Sci & Technol Beijing, Sch Chem & Biol Engn, Beijing 100083, Peoples R China

来源：

TOXINS | 2023年 / 15卷 / 08期

关键词：

microcystins; biodegradation; linearized microcystinase; purification; activity; CYANOBACTERIAL TOXINS; HETEROLOGOUS EXPRESSION; BACTERIAL-DEGRADATION; NODULARIN; GENE; RR; PATHWAY; LR; CHROMATOGRAPHY;

D O I：

10.3390/toxins15080494

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Hepatotoxic microcystins (MCs) are produced and released by the harmful bloom-forming cyanobacteria, which severely threaten drinking water safety and human health due to their high toxicity, widespread distribution, and structural stability. The linearized microcystinase (MlrB) further hydrolyses the poisonous linearized MCs produced by the microcystinase-catalysed MCs to form tetrapeptides. Here, the purification and activity of MlrB were investigated. The results showed that the linearized products generated by 12.5 mg/L MC-LR and MC-RR were removed by purified recombinant MlrB at a protein concentration of 1 mg/L within 30 min. The high catalytic activity of MlrB can be obtained via heterologous expression and affinity purification, which lays the foundation for further studies on the properties and mechanism of MCs biodegradation enzymes.

引用

页数：11

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