Synergistic effects of multiple rotors and hydrogen-bond interactions lead to sensitive near-infrared viscosity probes for live-cell microscopy

被引：31

作者：

Li, Dongyang ^{[1
]}

Shen, Tianruo ^{[2
]}

Xue, Xiaoqi ^{[3
]}

Chen, Weijie ^{[1
]}

Tao, Wenjun ^{[1
]}

Chi, Weijie ^{[4
]}

Liu, Sheng Hua ^{[1
]}

Tan, Ying ^{[3
]}

Liu, Xiaogang ^{[2
]}

Yin, Jun ^{[1
]}

机构：

[1] Cent China Normal Univ, Coll Chem, Int Joint Res Ctr Intelligent Biosensing Technol &, Natl Key Lab Green Pesticide, Wuhan 430079, Peoples R China

[2] Singapore Univ Technol & Design, Sci Math & Technol Cluster, Singapore 487372, Singapore

[3] Tsinghua Univ, Tsinghua Shenzhen Int Grad Sch, State Key Lab Chem Oncogen, Shenzhen 518055, Peoples R China

[4] Hainan Univ, Sch Sci, Dept Chem, Haikou 570228, Peoples R China

来源：

SCIENCE CHINA-CHEMISTRY | 2023年 / 66卷 / 08期

基金：

中国国家自然科学基金;

关键词：

fluorescent probe; high viscosity sensitivity; multiple rotors; hydrogen bonds; bioimaging; AGGREGATION-INDUCED EMISSION; MONITORING MITOCHONDRIAL VISCOSITY; FLUORESCENT-PROBE; INTRACELLULAR VISCOSITY; HEPTAMETHINE CYANINES; LYSOSOMAL VISCOSITY; DISEASE; DESIGN; MICE; DYES;

D O I：

10.1007/s11426-023-1661-6

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Changes in cellular viscosity are associated with various physiological processes and pathological conditions. To study these cellular processes and functions, highly sensitive fluorescent probes that detect subtle changes in viscosity are urgently needed but remain lacking. In this study, we present a series of viscosity-responsive near-infrared (NIR) fluorescent probes based on styrene-coated boron dipyrromethene (BODIPY). The probe modified with dimethylaminostyrene and piperazine at the two terminals of the BODIPY scaffold showed extremely high viscosity sensitivity values (x, around 1.54), with excellent performance for detecting viscosity below 20 cP. This outstanding property is attributed to the synergistic effects of multiple rotatable bonds and hydrogen-bond interactions. Additionally, this probe has been successfully deployed to monitor viscosity changes in various cellular compartments (i.e., cytoplasm) and processes (such as during autophagy). This work provides a rational molecular design strategy to construct fluorescent probes with high viscosity sensitivity for exploring cell functions.

引用

页码：2329 / 2338

页数：10

共 75 条

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