Single-Cell RNA Sequencing and Spatial Transcriptomics Reveal Pathogenesis of Meningeal Lymphatic Dysfunction after Experimental Subarachnoid Hemorrhage

被引：31

作者：

Wang, Xiaoyu ^{[1
,2
]}

Zhang, Anke ^{[1
,2
]}

Yu, Qian ^{[1
,2
]}

Wang, Zelin ^{[3
]}

Wang, Junjie ^{[4
]}

Xu, Penglei ^{[1
,2
]}

Liu, Yibo ^{[1
,2
]}

Lu, Jianan ^{[2
,4
]}

Zheng, Jingwei ^{[1
,2
]}

Li, Huaming ^{[1
,2
]}

Qi, Yangjian ^{[1
,2
]}

Zhang, Jiahao ^{[1
,2
]}

Fang, Yuanjian ^{[1
,2
]}

Xu, Shenbin ^{[5
]}

Zhou, Jingyi ^{[1
,2
]}

Wang, Kaikai ^{[1
,2
]}

Chen, Sheng ^{[1
,2
]}

Zhang, Jianmin ^{[1
,2
,6
,7
]}

机构：

[1] Zhejiang Univ, Affiliated Hosp 2, Sch Med, Dept Neurosurg, Hangzhou 310003, Zhejiang, Peoples R China

[2] Clin Res Ctr Neurol Dis Zhejiang Prov, Hangzhou 310003, Peoples R China

[3] Shuzhi Biotech LLC, Bioinformat, Guangzhou 510805, Peoples R China

[4] Zhejiang Univ, Affiliated Hosp 4, Int Inst Med, Dept Neurosurg,Sch Med, Yiwu 322000, Peoples R China

[5] Zhejiang Univ, Affiliated Hosp 2, Sch Med, Dept Gastrointestinal Surg, Hangzhou 310003, Peoples R China

[6] Zhejiang Univ, Brain Res Inst, Hangzhou 310003, Zhejiang, Peoples R China

[7] Zhejiang Univ, MOE Frontier Sci Ctr Brain Sci & Brain Machine Int, Hangzhou 310003, Zhejiang, Peoples R China

来源：

ADVANCED SCIENCE | 2023年 / 10卷 / 21期

基金：

中国国家自然科学基金;

关键词：

meningeal lymphatic; meningeal lymphatic endothelial cells; single-cell RNA sequencing; spatial transcriptome; subarachnoid hemorrhage; INTERSTITIAL FLUID; SYSTEM; BRAIN; CLEARANCE; ROLES; INFLAMMATION; VASCULATURE; HEMOGLOBIN; NEUTROPHIL; MIGRATION;

D O I：

10.1002/advs.202301428

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Subarachnoid hemorrhage (SAH) is a devastating subtype of stroke with high mortality and disability rate. Meningeal lymphatic vessels (mLVs) are a newly discovered intracranial fluid transport system and are proven to drain extravasated erythrocytes from cerebrospinal fluid into deep cervical lymph nodes after SAH. However, many studies have reported that the structure and function of mLVs are injured in several central nervous system diseases. Whether SAH can cause mLVs injury and the underlying mechanism remain unclear. Herein, single-cell RNA sequencing and spatial transcriptomics are applied, along with in vivo/vitro experiments, to investigate the alteration of the cellular, molecular, and spatial pattern of mLVs after SAH. First, it is demonstrated that SAH induces mLVs impairment. Then, through bioinformatic analysis of sequencing data, it is discovered that thrombospondin 1 (THBS1) and S100A6 are strongly associated with SAH outcome. Furthermore, the THBS1-CD47 ligand-receptor pair is found to function as a key role in meningeal lymphatic endothelial cell apoptosis via regulating STAT3/Bcl-2 signaling. The results illustrate a landscape of injured mLVs after SAH for the first time and provide a potential therapeutic strategy for SAH based on mLVs protection by disrupting THBS1 and CD47 interaction.

引用

页数：15

共 77 条

[1] Meningeal lymphatic vessels at the skull base drain cerebrospinal fluid [J].