Locus-specific detection of pseudouridine with CRISPR-Cas13a

被引：2
作者：
Sun, Mei [1 ]
Fang, Xin [1 ]
Lin, Bingqian [1 ]
Mo, Jing [1 ]
Wang, Fang [2 ]
Zhou, Xiang [1 ,3 ]
Weng, Xiaocheng [1 ]
机构：
[1] Wuhan Univ, Zhongnan Hosp, Dept Clin Lab, Minist Educ,Key Lab Biomed Polymers,Coll Chem & Mo, Wuhan 430072, Hubei, Peoples R China
[2] Wuhan Univ, Sch Pharmaceut Sci, Wuhan 430071, Peoples R China
[3] Wuhan Univ, Wuhan TaiKang Ctr Life & Med Sci, Wuhan 430071, Hubei, Peoples R China
来源：
CHEMICAL COMMUNICATIONS | 2024年 / 60卷 / 30期
基金：
中国国家自然科学基金;
关键词：
RNA PSEUDOURIDYLATION; GENE-EXPRESSION;
D O I：
10.1039/d4cc00179f
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
We combined the CRISPR-Cas13a system with CMC chemical labeling, developing an approach that enables precise identification of pseudouridine (psi) sites at specific loci within ribosomal RNA (rRNA), messenger RNA (mRNA) and small nuclear RNAs (snRNA). This method, with good efficiency and simplicity, detects psi sites through fluorescence measurement, providing a straightforward and fast validation for targeted psi sites of interest. A streamlined and efficient method enables precise detection of pseudouridine (psi) at specific loci, by leveraging CRISPR-Cas13a and CMC chemical labeling for immediate fluorescence analysis.
引用
收藏
页码：4088 / 4091
页数：4
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