Label-free quantitative detection and comparative analysis of lysine acetylation during the different life stages of Eimeria tenella

Proteome-wide lysine acetylation has been documentedin apicomplexanparasite Toxoplasma gondii and Plasmodium falciparum. Here, we conducted the firstlysine acetylome in unsporulated oocysts (USO), sporulated 7 h oocysts(SO 7h), sporulated oocysts (SO), sporozoites (S), and the secondgeneration merozoites (SMG) of Eimeria tenella through a 4D label-free quantitative technique. Altogether,8532 lysine acetylation sites on 2325 proteins were identified in E. tenella, among which 5445 sites on 1493 proteinswere quantified. In addition, 557, 339, 478, 248, 241, and 424 differentiallyexpressed proteins were identified in the comparisons SO7h vs USO,SO vs SO7h, SO vs USO, S vs SO, SMG vs S, and USO vs SMG, respectively.The bioinformatics analysis of the acetylome showed that the lysineacetylation is widespread on proteins of diverse functions. Moreover,the dynamic changes of lysine acetylome among E. tenella different life stages revealed significant regulation during thewhole process of E. tenella growthand stage conversion. This study provides a beginning for the investigationof the regulate role of lysine acetylation in E. tenella and may provide new strategies for anticoccidiosis drug and vaccinedevelopment. Raw data are publicly available at iProX with the dataset identifier PXD040368.