Oyster ferritin can efficiently alleviate ROS-mediated inflammation attributed to its unique micro-environment around three-fold channels

被引：3

作者：

Li, Han ^{[1
]}

Zang, Jiachen ^{[2
]}

Xia, Xiaoyu ^{[1
]}

Wang, Zhenyu ^{[1
]}

Wang, Li-Shu ^{[3
]}

EI-Seedi, Hesham R. ^{[4
]}

Xu, Xianbing ^{[1
]}

Zhao, Guanghua ^{[2
]}

Du, Ming ^{[1
]}

机构：

[1] Dalian Polytech Univ, Natl Engn Res Ctr Seafood, Sch Food Sci & Technol, Dalian 116034, Peoples R China

[2] China Agr Univ, Coll Food Sci & Nutr Engn, Beijing 100083, Peoples R China

[3] Med Coll Wisconsin, Dept Med, Div Hematol & Oncol, Milwaukee, WI 53226 USA

[4] Jiangsu Univ, Int Res Ctr Food Nutr & Safety, Zhenjiang 212013, Peoples R China

来源：

FREE RADICAL BIOLOGY AND MEDICINE | 2023年 / 204卷

关键词：

Ferritin; Point mutation; Structure; Oxidation activity; Fenton reaction; Reactive oxygen species; CHEMISTRY; STRESS;

D O I：

10.1016/j.freeradbiomed.2023.04.013

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The conversion of toxic Fe2+ into non-toxic Fe3+ stored in the inner cavity of ferritin nanocage could effectively reduce the occurrence of the Fenton reaction and inhibit the formation of harmful reactive oxygen species (ROS). In this study, we reveal that oyster ferritin (GF1) can rely on its high catalytic activity (7.7 times that of rHuHF) and high binding ability of Fe2+ (9.1 times that of rHuHF) to reduce the precursors of Fenton reaction, thus inhibiting the occurrence of Fenton reaction and slowing down reactive oxygen species-mediated inflammation. The above significant advantage of GF1 can be attributed to the Asp at the position 120th, which could increase the negatively charged area of three-fold channels from 37.8% (rHuHF) to 67.8% and then enhance its oxidation rate and ability of GF1. The findings are of great value in advancing novel nanoparticle drug design based on crystalline structure.

引用

页码：28 / 37

页数：10