Transcriptome Analysis Reveals Coexpression Networks and Hub Genes Involved in Papillae Development in Lilium auratum

被引:2
|
作者
Zhu, Yuntao [1 ]
Yang, Jie [1 ,2 ]
Liu, Xiaolin [1 ]
Sun, Tingting [1 ]
Zhao, Yiran [1 ]
Xiang, Fayun [2 ]
Chen, Feng [2 ]
He, Hengbin [1 ]
机构
[1] Beijing Forestry Univ, Sch Landscape Architecture, Beijing Key Lab Ornamental Plants Germplasm Innova, Natl Engn Res Ctr Floriculture,Beijing Lab Urban &, Beijing 100083, Peoples R China
[2] Hubei Acad Agr Sci, Ind Crops Inst, Wuhan 430064, Peoples R China
基金
中国国家自然科学基金;
关键词
Lilium auratum; petal papillae; anthocyanin accumulation; trichome development; MYB-bHLH-WD40; complex; ANTHOCYANIN BIOSYNTHESIS; TRICHOME DIFFERENTIATION; FLAVONOID BIOSYNTHESIS; ARABIDOPSIS; EXPRESSION; BIOCHEMISTRY; GENETICS; MYB; ACCUMULATION; PROTEINS;
D O I
10.3390/ijms25042436
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Lilium is a genus of important ornamental plants with many colouring pattern variations. Lilium auratum is the parent of Oriental hybrid lilies. A typical feature of L. auratum is the presence of red-orange special raised spots named papillae on the interior tepals. Unlike the usual raised spots, the papillae are slightly rounded or connected into sheets and usually have hairy tips. To elucidate the potential genes regulating papillae development in L. auratum, we performed high-throughput sequencing of its tepals at different stages. Genes involved in the flavonoid biosynthesis pathway were significantly enriched during the colouration of the papillae, and CHS, F3H, F3 ' H, FLS, DFR, ANS, and UFGT were significantly upregulated. To identify the key genes involved in the papillae development of L. auratum, we performed weighted gene coexpression network analysis (WGCNA) and further analysed four modules. In total, 51, 24, 1, and 6 hub genes were identified in four WGCNA modules, MEbrown, MEyellow, MEpurple, and MEred, respectively. Then, the coexpression networks were constructed, and important genes involved in trichome development and coexpressed with anthocyanin biosynthesis genes, such as TT8, TTG1, and GEM, were identified. These results indicated that the papillae are essentially trichomes that accumulate anthocyanins. Finally, we randomly selected 12 hub genes for qRT-PCR analysis to verify the accuracy of our RNA-Seq analysis. Our results provide new insights into the papillae development in L. auratum flowers.
引用
收藏
页数:16
相关论文
共 50 条
  • [21] Comparative transcriptome and coexpression network analysis reveals key pathways and hub candidate genes associated with sunflower (Helianthus annuus L.) drought tolerance
    Huimin Shi
    Jianhua Hou
    Dandan Li
    Haibo Hu
    Yanxia Wang
    Yang Wu
    Liuxi Yi
    BMC Plant Biology, 24
  • [22] Comparative transcriptome and coexpression network analysis reveals key pathways and hub candidate genes associated with sunflower (Helianthus annuus L.) drought tolerance
    Shi, Huimin
    Hou, Jianhua
    Li, Dandan
    Hu, Haibo
    Wang, Yanxia
    Wu, Yang
    Yi, Liuxi
    BMC PLANT BIOLOGY, 2024, 24 (01)
  • [23] Transcriptome analysis reveals hub genes in the hepatopancreas of Exopalaemon carinicauda in response to hypoxia and reoxygenation
    Wenjun Shi
    Pan Wang
    Runhao Hu
    Xihe Wan
    Hui Shen
    Hui Li
    Libao Wang
    Yi Qiao
    Ge Jiang
    Jie Cheng
    Zeyu Yang
    Aquaculture International, 2021, 29 : 1785 - 1811
  • [24] Transcriptome analysis reveals hub genes in the hepatopancreas of Exopalaemon carinicauda in response to hypoxia and reoxygenation
    Shi, Wenjun
    Wang, Pan
    Hu, Runhao
    Wan, Xihe
    Shen, Hui
    Li, Hui
    Wang, Libao
    Qiao, Yi
    Jiang, Ge
    Cheng, Jie
    Yang, Zeyu
    AQUACULTURE INTERNATIONAL, 2021, 29 (04) : 1785 - 1811
  • [25] Comparative transcriptome analysis reveals potential regulatory genes involved in the development and strength formation of maize stalks
    Cheng, Senan
    Qi, Youhui
    Lu, Dusheng
    Wang, Yancui
    Xu, Xitong
    Zhu, Deyun
    Ma, Dijie
    Wang, Shuyun
    Chen, Cuixia
    BMC PLANT BIOLOGY, 2025, 25 (01):
  • [26] Integrated analysis of transcriptome and proteome reveals the key genes and processes involved in ovarian development in Exopalaemon carinicauda
    Xing, Kefan
    Yan, Congcong
    Feng, Dandan
    Wu, Zixuan
    Sun, Yuying
    Zhang, Jiquan
    AQUACULTURE REPORTS, 2024, 37
  • [27] Transcriptome Analysis Reveals Candidate Genes Involved in Gibberellin-Induced Fruit Development in Rosa roxburghii
    Huang, Xiaolong
    Wu, Xiaoai
    Sun, Guilian
    Jiang, Yu
    Yan, Huiqing
    PLANTS-BASEL, 2023, 12 (19):
  • [28] Transcriptome analysis reveals key regulatory networks and genes involved in the acquisition of cold stress memory in pepper seedlings
    Li, Jian
    Yang, Ping
    Fu, Hongbo
    Li, Juan
    Wang, Yanzhuang
    Zhu, Keyan
    Yu, Jihua
    Li, Jie
    BMC PLANT BIOLOGY, 2024, 24 (01):
  • [29] Integrated transcriptomic analysis reveals hub genes involved in diagnosis and prognosis of pancreatic cancer
    Zhou, Yang-Yang
    Chen, Li-Ping
    Zhang, Yi
    Hu, Sun-Kuan
    Dong, Zhao-Jun
    Wu, Ming
    Chen, Qiu-Xiang
    Zhuang, Zhi-Zhi
    Du, Xiao-Jing
    MOLECULAR MEDICINE, 2019, 25 (01)
  • [30] Integrated transcriptomic analysis reveals hub genes involved in diagnosis and prognosis of pancreatic cancer
    Yang-Yang Zhou
    Li-Ping Chen
    Yi Zhang
    Sun-Kuan Hu
    Zhao-Jun Dong
    Ming Wu
    Qiu-Xiang Chen
    Zhi-Zhi Zhuang
    Xiao-Jing Du
    Molecular Medicine, 2019, 25