Evaluation of Performance of Different Culture Media in In Vitro Shoot Propagation of Local Grape Varieties

Turkiye is one of the most important countries for vineyard areas and grape production. Viticulture in Turkiye, which has suitable cultivation opportunities, has an old and deep-rooted history and is also considered as the native land of the vine. Viticulture, which has been carried out for many years, has bred a great wealth of grape genotypes. The presence of local varieties plays an important role in this diversity. It is important to develop an effective in vitro tissue culture system for the preservation and reproduction of local gene resources. This study was carried out to determine the optimal nutrient media for micropropagation of local grape (Vitis vinifera L.) germplasm. For micropropagation, three different basal media were tested, Murashige and Skoog (MS), McCown and Lloyd's woody plant medium (WPM) and Nitsch and Nitsch (NN), all three combined with 1 mg L-1 BAP. The highest shoot rate (93.33%) was observed on explants cultured on 'Sire' grape variety NN media, whereas this parameter was reduced on explants cultured on WPM and MS media (86.67%). The highest shoot rate (93.33%) was observed on explants cultured on 'Elma' grape variety NN media, whereas this parameter was reduced on explants cultured on WPM and MS media (40.00 and 46.67%, respectively). Depending on the variety duration of bud break (%), shooting rate (%), number of leaves (pieces/plantlet) and shoot length (cm) parameters, MS, NN and WPM nutrient media did show statistical differences.