A comprehensive investigation of lipid-transfer proteins from Cicer arietinum disentangles their role in plant defense against Helicoverpa armigera-infestation

被引：5

作者：

Saxena, Harshita ^{[1
,2
]}

Negi, Harshita ^{[1
,3
]}

Keshan, Radhika ^{[1
]}

Chitkara, Pragya ^{[4
]}

Kumar, Shailesh ^{[4
]}

Chakraborty, Amrita ^{[5
]}

Roy, Amit ^{[6
]}

Singh, Indrakant K. ^{[7
]}

Singh, Archana ^{[1
,8
]}

机构：

[1] Univ Delhi, Hansraj Coll, Dept Bot, Delhi, India

[2] Univ Georgia, Inst Plant Breeding Genet & Genom, Griffin, GA USA

[3] Univ South Carolina, Dept Biol Sci, Columbia, SC USA

[4] Bioinformat Lab, Natl Inst Plant Genome Res NIPGR, New Delhi, India

[5] Czech Univ Life Sci Prague, Fac Forestry & Wood Sci, EVA 4 0 Unit, Suchdol, Czech Republic

[6] Czech Univ Life Sci Prague, Fac Forestry & Wood Sci, Forest Mol Entomol Lab, EXTEMIT K,EVA 4 0, Suchdol, Czech Republic

[7] Univ Delhi, Deshbandhu Coll, Dept Zool, New Delhi, India

[8] Univ Delhi, Inst Eminence, Delhi Sch Climate Change & Sustainabil, Delhi, India

来源：

FRONTIERS IN GENETICS | 2023年 / 14卷

关键词：

lipid transfer proteins (LTPs); chickpea; Helicoverpa armigera; phylogenetic analysis; plant defense; herbivory; DIFFERENTIAL TRANSCRIPT ACCUMULATION; L; CHICKPEA; GENE; SEQUENCE; VISUALIZATION; RESISTANCE; JASMONATE; LOSSES; YIELD;

D O I：

10.3389/fgene.2023.1195554

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Lipid Transfer Proteins (LTPs) play a crucial role in synthesizing lipid barrier polymers and are involved in defense signaling during pest and pathogen attacks. Although LTPs are conserved with multifaceted roles in plants, these are not yet identified and characterized in Cicer arietinum. In this study, a genome-wide analysis of LTPs was executed and their physiochemical properties, biochemical function, gene structure analysis, chromosomal localization, promoter analysis, gene duplication, and evolutionary analysis were performed using in silico tools. Furthermore, tissue-specific expression analysis and gene expression analysis during pest attack was also conducted for the LTPs. A total of 48 LTPs were identified and named as CaLTPs. They were predicted to be small unstable proteins with "Glycolipid transfer protein" and "Alpha-Amylase Inhibitors, Lipid Transfer and Seed Storage" domains, that are translocated to the extracellular region. CaLTPs were predicted to possess 3-4 introns and were located on all the eight chromosomes of chickpea with half of the CaLTPs being localized on chromosomes 4, 5, and 6, and found to be closely related to LTPs of Arabidopsis thaliana and Medicago trancatula. Gene duplication and synteny analysis revealed that most of the CaLTPs have evolved due to tandem or segmental gene duplication and were subjected to purifying selection during evolution. The promoters of CaLTPs had development-related, phytohormone-responsive, and abiotic and biotic stress-related cis-acting elements. A few CaLTP transcripts exhibited differential expression in diverse tissue types, while others showed no/very low expression. Out of 20 jasmonate-regulated CaLTPs, 14 exhibited differential expression patterns during Helicoverpa armigera-infestation, indicating their role in plant defense response. This study identified and characterized CaLTPs from an important legume, C. arietinum, and indicated their involvement in plant defense against H. armigera-infestation, which can be further utilized to explore lipid signaling during plant-pest interaction and pest management.

引用

页数：18