Genomic Characterization of Uropathogenic Escherichia coli Isolates from Tertiary Hospitals in Riyadh, Saudi Arabia

被引：5

作者：

Aljohani, Rawan H. H. ^{[1
,2
]}

ElFeky, Dalia S. S. ^{[3
,4
]}

Alswaji, Abdulrahman A. A. ^{[1
]}

Alrashidi, Eisa ^{[1
]}

Okdah, Liliane ^{[1
]}

Alalwan, Bassam ^{[5
]}

Aljohani, Sameera M. M. ^{[1
,5
,6
]}

Balkhy, Hanan H. H. ^{[7
]}

Redhwan, Alya ^{[8
]}

Alghoribi, Majed F. F. ^{[1
,5
,6
]}

机构：

[1] King Abdullah Int Med Res Ctr, Infect Dis Res Dept, Riyadh 11481, Saudi Arabia

[2] Princess Nourah bint Abdulrahman Univ, Coll Sci, Dept Biol, Riyadh 11564, Saudi Arabia

[3] Princess Nourah bint Abdulrahman Univ, Coll Med, Dept Basic Med Sci, Riyadh 11564, Saudi Arabia

[4] Cairo Univ, Fac Med, Dept Med Microbiol & Immunol, Cairo 12613, Egypt

[5] King Abdulaziz Med City KAMC, Minist Natl Guard Hlth Affairs MNGHA, Dept Pathol & Lab Med, Riyadh 11426, Saudi Arabia

[6] King Saud bin Abdulaziz Univ Hlth Sci, Coll Sci & Hlth Profess, Dept Basic Sci, Riyadh 14611, Saudi Arabia

[7] WHO, CH-1211 Geneva, Switzerland

[8] Princess Nourah bint Abdulrahman Univ, Coll Hlth & Rehabil Sci, Dept Hlth, Riyadh 11564, Saudi Arabia

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2023年 / 24卷 / 08期

关键词：

uropathogenic E. coli; urinary tract infection; antimicrobial resistance; molecular typing; whole-genome sequence; extended-spectrum beta-lactamases; Saudi Arabia; SEQUENCE TYPE 131; ST131; MULTIRESISTANT;

D O I：

10.3390/ijms24087582

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Uropathogenic Escherichia coli (UPEC) is the most common cause of urinary tract infections (UTIs) in hospitalised and non-hospitalised patients. Genomic analysis was used to gain further insight into the molecular characteristics of UPEC isolates from Saudi Arabia. A total of 165 isolates were collected from patients with UTIs between May 2019 and September 2020 from two tertiary hospitals in Riyadh, Saudi Arabia. Identification and antimicrobial susceptibility testing (AST) were performed using the VITEK system. Extended-spectrum beta-lactamase (ESBL)-producing isolates (n = 48) were selected for whole genome sequencing (WGS) analysis. In silico analysis revealed that the most common sequence types detected were ST131 (39.6%), ST1193 (12.5%), ST73 (10.4%), and ST10 (8.3%). Our finding showed that bla(CTX- M-15) gene was detected in the majority of ESBL isolates (79.2%), followed by bla(CTX- M-27) (12.5%) and bla(CTX- M-8) (2.1%). ST131 carried bla(CTX- M-15) or bla(CTX- M-27), and all ST73 and ST1193 carried bla(CTX- M-15). The relatively high proportion of ST1193 in this study was notable as a newly emerged lineage in the region, which warrants further monitoring.

引用

页数：11

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