Label-Free Detection of T4 Polynucleotide Kinase Activity and Inhibition via Malachite Green Aptamer Generated from Ligation-Triggered Transcription

被引：4

作者：

Si, Jingyi ^{[1
]}

Zhou, Wei ^{[2
]}

Fang, Ying ^{[1
]}

Zhou, Da ^{[1
]}

Gao, Yifan ^{[1
]}

Yao, Qunyan ^{[1
]}

Shen, Xizhong ^{[1
]}

Zhu, Changfeng ^{[1
]}

机构：

[1] Fudan Univ, Zhongshan Hosp, Dept Gastroenterol & Hepatol, Shanghai 200032, Peoples R China

[2] Shanghai Yangpu Dist Ctr Dis Control & Prevent, Dept Sch & Nutr, Shanghai 200090, Peoples R China

来源：

BIOSENSORS-BASEL | 2023年 / 13卷 / 04期

关键词：

T4 polynucleotide kinase; malachite green aptamer; T7; transcription; COUPLED EXONUCLEASE REACTION; T7; RNA-POLYMERASE; SENSITIVE DETECTION; DNA; DNAZYME; ASSAY; NANOPARTICLE; ENZYME;

D O I：

10.3390/bios13040449

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Polynucleotide kinase (PNK) is a key enzyme that is necessary for ligation-based DNA repair. The activity assay and inhibitor screening for PNK may contribute to the prediction and improvement of tumor treatment sensitivity, respectively. Herein, we developed a simple, low-background, and label-free method for both T4 PNK activity detection and inhibitor screening by combining a designed ligation-triggered T7 transcriptional amplification system and a crafty light-up malachite green aptamer. Moreover, this method successfully detected PNK activity in the complex biological matrix with satisfactory outcomes, indicating its great potential in clinical practice.

引用

页数：12

共 44 条

[1] iSpinach: a fluorogenic RNA aptamer optimized for in vitro applications
Autour, Alexis
Westhof, Eric
Ryckelynck, Michael
[J]. NUCLEIC ACIDS RESEARCH, 2016, 44 (06) : 2491 - 2500
[2] Targeting the DNA Damage Response for Cancer Therapy by Inhibiting the Kinase Wee1
Bukhari, Amirali B.
Chan, Gordon K.
Gamper, Armin M.
[J]. FRONTIERS IN ONCOLOGY, 2022, 12
[3] One-step highly sensitive florescence detection of T4 polynucleotide kinase activity and biological small molecules by ligation-nicking coupled reaction-mediated signal amplification
Chen, Feng
Zhao, Yongxi
Qi, Lin
Fan, Chunhai
[J]. BIOSENSORS & BIOELECTRONICS, 2013, 47 : 218 - 224
[4] New Insights into Mechanisms of Cisplatin Resistance: From Tumor Cell to Microenvironment
Chen, Shang-Hung
Chang, Jang-Yang
[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2019, 20 (17)
[5] Label-free and sensitive detection of T4 polynucleotide kinase activity via coupling DNA strand displacement reaction with enzymatic-aided amplification
Cheng, Rui
Tao, Mangjuan
Shi, Zhilu
Zhang, Xiafei
Jin, Yan
Li, Baoxin
[J]. BIOSENSORS & BIOELECTRONICS, 2015, 73 : 138 - 145
[6] An ultrasensitive electrochemical biosensor for polynucleotide kinase assay based on gold nanoparticle-mediated lambda exonuclease cleavage-induced signal amplification
Cui, Lin
Li, Yueying
Lu, Mengfei
Tang, Bo
Zhang, Chun-yang
[J]. BIOSENSORS & BIOELECTRONICS, 2018, 99 : 1 - 7
[7] Detection of T4 Polynucleotide Kinase via Allosteric Aptamer Probe Platform
Gao, Mingxuan
Guo, Jingjing
Song, Yanling
Zhu, Zhi
Yang, Chaoyong James
[J]. ACS APPLIED MATERIALS & INTERFACES, 2017, 9 (44) : 38356 - 38363
[8] Label-free luminescence switch-on detection of T4 polynucleotide kinase activity using a G-quadruplex-selective probe
He, Hong-Zhang
Leung, Ka-Ho
Wang, Wei
Chan, Daniel Shiu-Hin
Leung, Chung-Hang
Ma, Dik-Lung
[J]. CHEMICAL COMMUNICATIONS, 2014, 50 (40) : 5313 - 5315
[9] Sensitive electrochemical assay for T4 polynucleotide kinase activity based on dual-signaling amplification coupled with exonuclease reaction
Hou, Ting
Wang, Xiuzhong
Liu, Xinglu
Pan, Chao
Li, Feng
[J]. SENSORS AND ACTUATORS B-CHEMICAL, 2014, 202 : 588 - 593
[10] Sensitive detection of T4 polynucleotide kinase activity based on coupled exonuclease reaction and nicking enzyme-assisted fluorescence signal amplification
Hou, Ting
Wang, Xiuzhong
Lu, Tingting
Liu, Xiaojuan
Li, Feng
[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2014, 406 (12) : 2943 - 2948

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