Influence of Ascorbic Acid as a Growth and Differentiation Factor on Dental Stem Cells Used in Regenerative Endodontic Therapies

被引:7
作者
Diederich, Antje [1 ]
Fruend, Hanna Juliane [2 ]
Trojanowicz, Bogusz [2 ]
Navarrete Santos, Alexander [3 ]
Nguyen, Anh Duc [1 ,4 ]
Hoang-Vu, Cuong [2 ]
Gernhardt, Christian Ralf [1 ]
机构
[1] Martin Luther Univ Halle Wittenberg, Univ Outpatient Clin Conservat Dent & Periodontol, D-06112 Halle, Germany
[2] Martin Luther Univ Halle Wittenberg, Univ Med Ctr Halle, Dept Visceral Vasc & Endocrine Surg, D-06120 Halle, Germany
[3] Martin Luther Univ Halle Wittenberg, Ctr Med Basic Res, D-06108 Halle, Germany
[4] Private Dent Practice Dr Juliane Gernhardt, D-06120 Halle, Germany
关键词
ascorbic acid; dental pulp stem cells; regenerative endodontic procedures; stem cell markers; PULP; PROLIFERATION; EXPRESSION; APOPTOSIS; BIOLOGY;
D O I
10.3390/jcm12031196
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Background: Vitamin C is one of the major extracellular nonenzymatic antioxidants involved in the biosynthesis of collagen. It promotes the growth of fibroblasts, wound healing processes, and enhances the survival and differentiation of osteoblasts. The potential effects of ascorbic acid on human dental pulp cells (DPC) and the cells of the apical papilla (CAP) used in actual regenerative endodontic procedures remain largely unknown. In this study, we investigated the possible employment of ascorbic acid in the differentiation and regenerative therapies of DPC and CAP. Methods: Nine extracted human wisdom teeth were selected for this study. Subpopulations of stem cells within DPC and CAP were sorted with the mesenchymal stem cell marker STRO-1, followed by treatments with different concentrations (0 mM, 0.1 mM, 0.5 mM, and 1.0 mM) of ascorbic acid (AA), RT-PCR, and Western blot analysis. Results: FACS analysis revealed the presence of cell subpopulations characterized by a strong expression of mesenchymal stem cell marker STRO-1 and dental stem cell markers CD105, CD44, CD146, CD90, and CD29. Treatment of the cells with defined amounts of AA revealed a markedly increased expression of proliferation marker Ki-67, especially in the concentration range between 0.1 mM and 0.5 mM. Further investigations demonstrated that treatment with AA led to significantly increased expression of common stem cell markers OCT4, Nanog, and Sox2. The most potent proliferative and expressional effects of AA were observed in the concentration of 0.1 mM. Conclusions: AA might be a novel and potent growth promoter of human dental cells. Increasing the properties of human dental pulp cells and the cells of the apical papilla using AA could be a useful factor for further clinical developments of regenerative endodontic procedures.
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页数:9
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