Preparation of γ-linolenic acid rich triacylglycerol from borage oil via a two-step lipase-catalyzed reaction

被引：4

作者：

Yoon, Hui Su ^{[1
]}

Lee, Yu Jin ^{[1
]}

Kim, Byung Hee ^{[2
]}

Kim, Yangha ^{[3
]}

Kim, In-Hwan ^{[1
,4
]}

机构：

[1] Korea Univ, Grad Sch, Dept Integrated Biomed & Life Sci, Transdisciplinary Major Learning Hlth Syst, Seoul, South Korea

[2] Sookmyung Womens Univ, Dept Food & Nutr, Seoul, South Korea

[3] Ewha Womans Univ, Dept Nutr Sci & Food Management, Seoul, South Korea

[4] Korea Univ, Grad Sch, Dept Integrated Biomed & Life Sci, 145 Anam Ro, Seoul 02841, South Korea

来源：

JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY | 2023年 / 100卷 / 03期

基金：

新加坡国家研究基金会;

关键词：

borage oil; Candida rugosa lipase; enrichment; glyceride; triglyceride (TAG); gamma-linolenic acid (GLA); POLYUNSATURATED FATTY-ACIDS; ENRICHED TRIACYLGLYCEROL; EICOSAPENTAENOIC ACID; DOCOSAHEXAENOIC ACID; ETHYL-ESTER; FISH-OIL; ESTERIFICATION; GLYCEROLYSIS; SOLVENT; WATER;

D O I：

10.1002/aocs.12678

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

gamma-Linolenic acid (GLA) rich triacylglycerol (TAG) was successfully synthesized from glyceride, instead of glycerol, and fatty acid (FA) via Lipozyme TL IM-catalyzed esterification as a novel strategy. In the first step, GLA was enriched into glyceride fraction from borage oil by Candida rugosa lipase-catalyzed hydrolysis. The glyceride was separated from the reaction mixture by molecular distillation. GLA was enriched from 20.64% in borage oil to 45.94% in the glyceride fraction under optimum conditions. In the second step, the Lipozyme TL IM-catalyzed synthesis of TAG was carried out with the glyceride, and the FA obtained by saponification of a portion of the glyceride. The optimum conditions were the temperature of 50?, the enzyme loading of 10%, and the vacuum level of 20 mmHg, respectively. The maximum TAG content of approximately 92% was achieved after 12 h under the optimum conditions.

引用

页码：215 / 224

页数：10

共 51 条

[11] Enhancement of both reaction yield and rate of synthesis of structured triacylglycerol containing eicosapentaenoic acid under vacuum with water activity control [J].