Evaluation of nanoparticle albumin-bound paclitaxel loaded macrophages for glioblastoma treatment based on a microfluidic chip

被引：4

作者：

Xie, Zuorun ^{[1
]}

Ye, Junyi ^{[1
]}

Gao, Xinghua ^{[2
]}

Chen, Hang ^{[3
]}

Chen, Maosong ^{[1
]}

Lian, Jiangfang ^{[3
]}

Ma, Jingyun ^{[3
]}

Wang, Hongcai ^{[1
]}

机构：

[1] Ningbo Univ, Affiliated Lihuili Hosp, Dept Neurosurg, Ningbo, Zhejiang, Peoples R China

[2] Shanghai Univ, Mat Genome Inst, Shanghai, Peoples R China

[3] Ningbo Univ, Affiliated Lihuili Hosp, Ningbo Inst Innovat Combined Med & Engn, Ningbo, Zhejiang, Peoples R China

来源：

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY | 2024年 / 12卷

关键词：

microfluidic chip; glioblastoma; macrophage-based carrier; drug delivery; nanoparticle albumin-bound paclitaxel; DELIVERY; GLIOMA;

D O I：

10.3389/fbioe.2024.1361682

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Introduction: Glioblastoma (GBM) is a primary brain malignancy with a dismal prognosis and remains incurable at present. In this study, macrophages (M phi) were developed to carry nanoparticle albumin-bound paclitaxel (nab-PTX) to form nab-PTX/M phi. The aim of this study is to use a GBM-on-a-chip to evaluate the anti-GBM effects of nab-PTX/M phi.Methods: In this study, we constructed nab-PTX/M phi by incubating live M phi with nab-PTX. We developed a microfluidic chip to co-culture GBM cells and human umbilical vein endothelial cells, mimicking the simplified blood-brain barrier and GBM. Using a syringe pump, we perform sustainable perfusion of nutrient media. To evaluate the anti-GBM effects nab-PTX/M phi, we treated the GBM-on-a-chip model with nab-PTX/M phi and investigated GBM cell proliferation, migration, and spheroid formation.Results: At the chosen concentration, nab-PTX did not significantly affect the viability, chemotaxis and migration of M phi. The uptake of nab-PTX by M phi occurred within 1 h of incubation and almost reached saturation at 6 h. Additionally, nab-PTX/M phi exhibited the M1 phenotype, which inhibits tumor progression. Following phagocytosis, M phi were able to release nab-PTX, and the release of nab-PTX by M phi had nearly reached its limit at 48 h. Compared with control group and blank M phi group, individual nab-PTX group and nab-PTX/M phi group could inhibit tumor proliferation, invasion and spheroid formation. Meanwhile, the anti-GBM effect of nab-PTX/M phi was more significant than nab-PTX.Discussion: Our findings demonstrate that nab-PTX/M phi has a significant anti-GBM effect compared to individual nab-PTX or M phi administration, suggesting M phi as potential drug delivery vectors for GBM therapy. Furthermore, the developed GBM-on-a-chip model provides a potential ex vivo platform for innovative cell-based therapies and tailored therapeutic strategies for GBM.

引用

页数：15

共 50 条

[41] Repeated blood-brain barrier opening with an implantable ultrasound device for delivery of albumin-bound paclitaxel in patients with recurrent glioblastoma: a phase 1 trial [J].

Sonabend, Adam M. ;

Gould, Andrew ;

Amidei, Christina ;

Ward, Rachel ;

Schmidt, Karyn A. ;

Zhang, Daniel Y. ;

Gomez, Cristal ;

Bebawy, John F. ;

Liu, Benjamin P. ;

Bouchoux, Guillaume ;

Desseaux, Carole ;

Helenowski, Irene B. ;

Lukas, Rimas, V ;

Dixit, Karan ;

Kumthekar, Priya ;

Arrieta, Victor A. ;

Lesniak, Maciej S. ;

Carpentier, Alexandre ;

Zhang, Hui ;

Muzzio, Miguel ;

Canney, Michael ;

Stupp, Roger .

LANCET ONCOLOGY, 2023, 24 (05) :509-522

[42] De-escalated neoadjuvant therapy with nanoparticle albumin-bound paclitaxel and trastuzumab for low-risk pure HER2 breast cancer [J].

Satoru Tanaka ;

Nobuki Matsunami ;

Hirotaka Morishima ;

Naofumi Oda ;

Tsutomu Takashima ;

Satoru Noda ;

Shinichiro Kashiwagi ;

Yukie Tauchi ;

Yuka Asano ;

Kosei Kimura ;

Hiroya Fujioka ;

Risa Terasawa ;

Kanako Kawaguchi ;

Ayana Ikari ;

Takashi Morimoto ;

Shintaro Michishita ;

Toshihiro Kobayashi ;

Junna Sakane ;

Toshikatsu Nitta ;

Nayuko Sato ;

Norihiro Hokimoto ;

Yukihiro Nishida ;

Mitsuhiko Iwamoto .

Cancer Chemotherapy and Pharmacology, 2019, 83 :1099-1104

[43] De-escalated neoadjuvant therapy with nanoparticle albumin-bound paclitaxel and trastuzumab for low-risk pure HER2 breast cancer [J].

Tanaka, Satoru ;

Matsunami, Nobuki ;

Morishima, Hirotaka ;

Oda, Naofumi ;

Takashima, Tsutomu ;

Noda, Satoru ;

Kashiwagi, Shinichiro ;

Tauchi, Yukie ;

Asano, Yuka ;

Kimura, Kosei ;

Fujioka, Hiroya ;

Terasawa, Risa ;

Kawaguchi, Kanako ;

Ikari, Ayana ;

Morimoto, Takashi ;

Michishita, Shintaro ;

Kobayashi, Toshihiro ;

Sakane, Junna ;

Nitta, Toshikatsu ;

Sato, Nayuko ;

Hokimoto, Norihiro ;

Nishida, Yukihiro ;

Iwamoto, Mitsuhiko .

CANCER CHEMOTHERAPY AND PHARMACOLOGY, 2019, 83 (06) :1099-1104

[44] Meta-analysis of nanoparticle albumin-bound paclitaxel used as neoadjuvant chemotherapy for operable breast cancer based on individual patient data (JBCRG-S01 study) [J].

Futamura, Manabu ;

Oba, Mari ;

Masuda, Norikazu ;

Bando, Hiroko ;

Okada, Morihito ;

Yamamoto, Yutaka ;

Kin, Takanori ;

Saeki, Toshiaki ;

Nagashima, Takeshi ;

Kuwayama, Takashi ;

Toh, Uhi ;

Hirano, Akira ;

Inokuchi, Masafumi ;

Yamagami, Kazuhiko ;

Mizuno, Yutaka ;

Kojima, Yasuyuki ;

Nakayama, Takahiro ;

Yasojima, Hiroyuki ;

Ohno, Shinji .

BREAST CANCER, 2021, 28 (05) :1023-1037

[45] Weekly nanoparticle albumin-bound paclitaxel in combination with cisplatin versus weekly solvent-based paclitaxel plus cisplatin as first-line therapy in Chinese patients with advanced esophageal squamous cell carcinoma [J].

Wang, Hai-ying ;

Yao, Zhi-hua ;

Tang, Hong ;

Zhao, Yan ;

Zhang, Xiao-san ;

Yao, Shu-na ;

Yang, Shu-jun ;

Liu, Yan-yan .

ONCOTARGETS AND THERAPY, 2016, 9 :5663-5669

[46] Meta-analysis of nanoparticle albumin-bound paclitaxel used as neoadjuvant chemotherapy for operable breast cancer based on individual patient data (JBCRG-S01 study) [J].

Manabu Futamura ;

Mari Oba ;

Norikazu Masuda ;

Hiroko Bando ;

Morihito Okada ;

Yutaka Yamamoto ;

Takanori Kin ;

Toshiaki Saeki ;

Takeshi Nagashima ;

Takashi Kuwayama ;

Uhi Toh ;

Akira Hirano ;

Masafumi Inokuchi ;

Kazuhiko Yamagami ;

Yutaka Mizuno ;

Yasuyuki Kojima ;

Takahiro Nakayama ;

Hiroyuki Yasojima ;

Shinji Ohno .

Breast Cancer, 2021, 28 :1023-1037

[47] Paclitaxel/methotrexate co-loaded PLGA nanoparticles in glioblastoma treatment: Formulation development and in vitro antitumor activity evaluation [J].

Madani, Fatemeh ;

Esnaashari, Seyedeh Sara ;

Bergonzi, Maria Camilla ;

Webster, Thomas J. ;

Younes, Husam M. ;

Khosravani, Masood ;

Adabi, Mahdi .

LIFE SCIENCES, 2020, 256

[48] Curcumin-loaded nanoparticle based on poloxamer188 for glioma treatment: Synthesis, characterization and in vitro evaluation [J].

Hou, Xueyan ;

Xu, Jingjing ;

He, Sisi ;

Bai, Jintao ;

Guan, Yalin ;

Pan, Xiaofei ;

Yang, Jiake ;

Zhang, Nan ;

Yang, Shang ;

Hu, Jie ;

Yang, Xue .

POLYMERS FOR ADVANCED TECHNOLOGIES, 2023, 34 (09) :2993-3002

[49] The Efficacy of Combined Cisplatin and Nanoparticle Albumin-Bound Paclitaxel in a Stage IV Pancreatic Squamous Cell Carcinoma Patient With a Somatic BRCA2 Mutation: A Case Report [J].

Huang, Xiaozhun ;

Wang, Chunling ;

Ma, Teng ;

Huang, Zhangkan ;

Zhou, Houhong ;

Xu, Lin ;

Zhang, Renjie ;

Zhao, Jianjun ;

Zhang, Yefan ;

Huang, Zhen ;

Shao, Lin ;

Wang, Yang ;

Yang, Fan ;

Che, Xu .

FRONTIERS IN ONCOLOGY, 2021, 11

[50] Direct comparison of two albumin-based paclitaxel-loaded nanoparticle formulations: Is the crosslinked version more advantageous? [J].

Li, Chunlei ;

Li, Yanhui ;

Gao, Yuqing ;

Wei, Na ;

Zhao, Xi ;

Wang, Caixia ;

Li, Yongfeng ;

Xiu, Xian ;

Cui, Jingxia .

INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2014, 468 (1-2) :15-25

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