An accurate spectrophotometric method for chitosan quantification

被引：0

作者：

Gonzalez-Davis, Oscar ^{[1
]}

Betanzo, Itandehui ^{[1
]}

Vazquez-Duhalt, Rafael ^{[1
]}

机构：

[1] Univ Nacl Autonoma Mexico, Ctr Nanociencias & Nanotecnol, Baja California 22860, Mexico

来源：

BIOLOGY METHODS & PROTOCOLS | 2023年 / 8卷 / 01期

关键词：

anthrone; carbohydrate; chitosan determination; deamination; NANOPARTICLES; ASSAY; HYDROLYSIS; DELIVERY;

D O I：

10.1093/biomethods/bpad036

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Chitosan is a biopolymer obtained from chitin, one of the most abundant biopolymers in nature. Numerous applications of chitosan are well known in the biomedical, environmental, and industrial fields, and the potential applications are considerable. This work reports a new spectrophotometric method to determine chitosan concentration accurately. The method is based on the deamination of chitosan by nitrite in acidic conditions, followed by a carbohydrate determination by the anthrone reagent.

引用

页数：4

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