Generation of a gene-edited H9 embryonic stem cell line carrying a DOX-inducible NGN2 expression cassette in the CLYBL locus

被引：2
|
作者：
Miellet, Sara [1 ]
St Clair-Glover, Mitchell [1 ]
Maddock, Marnie [1 ]
Dottori, Mirella [1 ]
机构：
[1] Univ Wollongong, Sch Med Indigenous & Hlth Sci, Mol Horizons, Wollongong, Australia
来源：
STEM CELL RESEARCH | 2024年 / 75卷
基金：
澳大利亚研究理事会;
关键词：
NEURONS;
D O I：
10.1016/j.scr.2024.103312
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
The pro -neural transcription factor neurogenin-2 (NGN2) possesses the ability to rapidly and effectively transform stem cells into fully operational neurons. Here we report the successful generation of a modified H9 human embryonic H9 stem cell line containing a doxycycline (DOX) inducible NGN2 expression construct featuring a floxed Blasticidin/mApple selection module in the safe -harbor locus CLYBL. This cell line retains its pluripotent state in the absence of DOX, yet readily transitions into a neuronal state upon DOX introduction.
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页数：5
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