16S rRNA gene sequencing reveals the effect of fluoxetine on gut microbiota in chronic unpredictable stress-induced depressive-like rats

被引：3
作者：
Dong, Hui [1 ]
Tang, Xiaowei [1 ]
Ye, Jie [1 ]
Xiao, Wenhuan [1 ]
机构：
[1] Yangzhou Univ, Wutaishan Hosp, Teaching Hosp, Yangzhou, Jiangsu, Peoples R China
来源：
ANNALS OF GENERAL PSYCHIATRY | 2023年 / 22卷 / 01期
关键词：
Depression; Fluoxetine; Gut microbiota; Illumina sequencing; 16S rRNA; PROBIOTIC PROPERTIES; GABA CONCENTRATIONS; LACTOBACILLUS; SEROTONIN; ANXIETY; BRAIN; EXPRESSION; INGESTION; BACTERIA; PLACEBO;
D O I：
10.1186/s12991-023-00458-x
中图分类号：
R749 [精神病学];
学科分类号：
100205 ;
摘要：
Objectives Gut microbiota is relevant to the pathogenesis of mental disorders including depression. This study aimed to investigate the influence of fluoxetine (FLX) on the gut microbiota in rats with Chronic Unpredictable Mild Stresses (CUMS)-induced depression. Results We confirmed that the 28-day CUMS-induced depression rat model. Chronic FLX administration weakly improved depressive-like behaviors in rats. Illumina 16S rRNA gene sequencing on rat feces showed CUMS increased the relative abundance of Firmicutes (60.31% vs. 48.09% in Control, p < 0.05) and Lactobacillus genus (21.06% vs. 6.82% in control, p < 0.05); FLX and CUMS increased Bacilli class (20.00% similar to 24.08% vs. 10.31% in control, p < 0.05). Conclusion Collectively, our study showed that both CUMS and FLX changed the compositions of gut microbiota in rats. FLX and CUMS distinctly regulated the gut microbiota in depressed rats.
引用
收藏
页数：10
相关论文
共 34 条
[1]   Possible association of Bifidobacterium and Lactobacillus in the gut microbiota of patients with major depressive disorder [J].
Aizawa, Emiko ;
Tsuji, Hirokazu ;
Asahara, Takashi ;
Takahashi, Takuya ;
Teraishi, Toshiya ;
Yoshida, Sumiko ;
Ota, Miho ;
Koga, Norie ;
Hattori, Kotaro ;
Kunugi, Hiroshi .
JOURNAL OF AFFECTIVE DISORDERS, 2016, 202 :254-257
[2]  
American Psychiatric Association, 2022, Diagnostic and Statistical Manual of Mental Disorders Text Revision, V5th ed., text rev.
[3]   The glucocorticoid receptor: Pivot of depression and of antidepressant treatment? [J].
Anacker, Christoph ;
Zunszain, Patricia A. ;
Carvalho, Livia A. ;
Pariante, Carmine M. .
PSYCHONEUROENDOCRINOLOGY, 2011, 36 (03) :415-425
[4]  
[Anonymous], 2013, EUR CHEM B, DOI DOI 10.17628/ECB.2013.2.373-382
[5]   Efficacy and tolerability of tricyclic antidepressants and SSRIs compared with placebo for treatment of depression in primary care: A meta-analysis [J].
Arroll, B ;
Macgillivray, S ;
Ogston, S ;
Reid, I ;
Sullivan, F ;
Williams, B ;
Crombie, I .
ANNALS OF FAMILY MEDICINE, 2005, 3 (05) :449-456
[6]  
Avila J, 2010, REV CIENT-FAC CIEN V, V20, P161
[7]   γ-Aminobutyric acid production by culturable bacteria from the human intestine [J].
Barrett, E. ;
Ross, R. P. ;
O'Toole, P. W. ;
Fitzgerald, G. F. ;
Stanton, C. .
JOURNAL OF APPLIED MICROBIOLOGY, 2012, 113 (02) :411-417
[8]   Increased brain GABA concentrations following acute administration of a selective serotonin reuptake inhibitor [J].
Bhagwagar, Z ;
Wylezinska, M ;
Taylor, M ;
Jezzard, P ;
Matthews, PM ;
Cowen, PJ .
AMERICAN JOURNAL OF PSYCHIATRY, 2004, 161 (02) :368-370
[9]   Ingestion of Lactobacillus strain regulates emotional behavior and central GABA receptor expression in a mouse via the vagus nerve [J].
Bravo, Javier A. ;
Forsythe, Paul ;
Chew, Marianne V. ;
Escaravage, Emily ;
Savignac, Helene M. ;
Dinan, Timothy G. ;
Bienenstock, John ;
Cryan, John F. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2011, 108 (38) :16050-16055
[10]   Local potentiation of excitatory synapses by serotonin and its alteration in rodent models of depression [J].
Cai, Xiang ;
Kallarackal, Angy J. ;
Kvarta, Mark D. ;
Goluskin, Sasha ;
Gaylor, Kaitlin ;
Bailey, Aileen M. ;
Lee, Hey-Kyoung ;
Huganir, Richard L. ;
Thompson, Scott M. .
NATURE NEUROSCIENCE, 2013, 16 (04) :464-U135
1234