Profiling of metabolites, proteins, and protein phosphorylation in silica-exposed BEAS-2B epithelial cells

被引：2

作者：

Chen, Jin ^{[1
]}

Wang, Hanshi ^{[2
]}

Gao, Hongzhi ^{[1
]}

Zeng, Yiming ^{[3
]}

机构：

[1] Fujian Med Univ, Affiliated Hosp 2, Clin Ctr Mol Diag & Therapy, Quanzhou, Fujian, Peoples R China

[2] Fujian Med Univ, Affiliated Hosp 2, Dept Orthoped, Quanzhou, Fujian, Peoples R China

[3] Fujian Med Univ, Affiliated Hosp 2, Dept Resp Med, Quanzhou, Fujian, Peoples R China

来源：

PLOS ONE | 2023年 / 18卷 / 04期

基金：

中国国家自然科学基金;

关键词：

CRYSTALLINE SILICA; UNITED-STATES; ACTIVATION; MORTALITY; UPDATE; GENE;

D O I：

10.1371/journal.pone.0273034

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Silicosis is an uncurable occupational disease induced by crystalline silica. Increased prevalence of silicosis has resulted in the increased need for development of treatment options. Although macrophages respond first to silica, epithelial cells are also involved in silicosis. However, changes in protein and metabolite levels have not been reported simultaneously. We used mass spectrometry to profile changes in metabolites, proteins, and phosphorylation in silica-exposed BEAS-2B epithelial cells. Silica exposure increased TCA cycle, alanine, aspartate and glutamate metabolism, and aerobic glycolysis activity. In addition, protein levels in the endoplasmic reticulum were significantly altered, and phosphorylation of MAPK signaling proteins was increased. The results of this study increased understanding the role of epithelial cells in silicosis.

引用

页数：13

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