Surface functionalization modification of ultra-hydrophilic magnetic spheres with mesoporous silica for specific identification of glycopeptides in serum exosomes

被引:6
作者
Yi, Linhua [1 ]
Wang, Baichun [2 ]
Feng, Quanshou [2 ]
Yan, Yinghua [1 ,2 ]
Ding, Chuan-Fan [1 ,2 ]
Mao, Haijiao [1 ]
机构
[1] Ningbo Univ, Affiliated Hosp, Med Sch, Dept Orthopaed Surg, Ningbo 315020, Zhejiang, Peoples R China
[2] Ningbo Univ, Sch Mat Sci & Chem Engn, Key Lab Adv Mass Spectrometry & Mol Anal Zhejiang, Ningbo 315211, Zhejiang, Peoples R China
关键词
Fe3O4-CG@mSiO(2); Hydrophilic mesoporous nanospheres; Glycopeptide enrichment; Exosomes; Nano-LC-MS/MS; HIGHLY EFFICIENT ENRICHMENT; ONE-STEP FUNCTIONALIZATION; L-CYSTEINE; NANOPARTICLES; FACILE; ACID;
D O I
10.1007/s00216-023-04575-0
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Protein glycosylation of human serum exosomes can reveal significant physiological information, and the development of large-scale identification strategies is crucial for the in-depth investigation of the serum exosome glycoproteome. In this study, using surface functionalization techniques, an ultra-hydrophilic mesoporous silica magnetic nanosphere (denoted as Fe3O4-CG@mSiO(2)) was synthesized for the quick and accurate detection of glycopeptides from HRP digests. The Fe3O4-CG@mSiO(2) nanospheres demonstrated outstanding enrichment capability, high sensitivity (5 amol/mu L), good size exclusion effect (HRP digests/BSA proteins, 1:10,000), stable reusability (at least 10 times), and an excellent recovery rate (108.6 +/- 5.5%). Additionally, after enrichment by Fe3O4-CG@mSiO(2), 156 glycopeptides assigned to 64 proteins derived from human serum exosomes were successfully identified, which demonstrates that the nanospheres have great potential for the research of the large-scale serum exosome glycoproteome.
引用
收藏
页码:1741 / 1749
页数:9
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