Parallelized Acquisition of Orbitrap and Astral Analyzers Enables High-Throughput Quantitative Analysis

被引：65

作者：

Stewart, Hamish ^{[1
]}

Grinfeld, Dmitry ^{[1
]}

Giannakopulos, Anastassios ^{[1
]}

Petzoldt, Johannes ^{[1
]}

Shanley, Toby ^{[1
]}

Garland, Matthew ^{[1
]}

Denisov, Eduard ^{[1
]}

Peterson, Amelia ^{[1
]}

Damoc, Eugen ^{[1
]}

Zeller, Martin ^{[1
]}

Arrey, Tabiwang ^{[1
]}

Pashkova, Anna ^{[1
]}

Renuse, Santosh ^{[2
]}

Hakimi, Amirmansoor ^{[2
]}

Kuhn, Andreas ^{[1
]}

Biel, Matthias ^{[1
]}

Kreutzmann, Arne ^{[1
]}

Hagedorn, Bernd ^{[1
]}

Colonius, Immo ^{[1
]}

Schutz, Adrian ^{[1
]}

Stefes, Arne ^{[1
]}

Dwivedi, Ankit ^{[1
]}

Mourad, Daniel ^{[1
]}

Hoek, Max ^{[1
]}

Reitemeier, Bastian ^{[1
]}

Cochems, Philipp ^{[1
,2
]}

Kholomeev, Alexander ^{[1
]}

Ostermann, Robert ^{[1
]}

Quiring, Gregor ^{[1
]}

Ochmann, Maximilian ^{[1
]}

Mohring, Sascha ^{[1
]}

Wagner, Alexander ^{[1
]}

Petker, Andre ^{[1
]}

Kanngiesser, Sebastian ^{[1
]}

Wiedemeyer, Michael ^{[1
]}

Balschun, Wilko ^{[1
]}

Hermanson, Daniel ^{[2
]}

Zabrouskov, Vlad ^{[2
]}

Makarov, Alexander ^{[1
]}

Hock, Christian ^{[1
]}

机构：

[1] Thermo Fisher Sci, D-28199 Bremen, Germany

[2] Thermo Fisher Sci, San Jose, CA 95134 USA

来源：

ANALYTICAL CHEMISTRY | 2023年 / 95卷 / 42期

关键词：

MASS-SPECTROMETER; PROTEOME COVERAGE; QUADRUPOLE; IDENTIFICATION; DRAFT;

D O I：

10.1021/acs.analchem.3c02856

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The growing trend toward high-throughput proteomics demands rapid liquid chromatography-mass spectrometry (LC-MS) cycles that limit the available time to gather the large numbers of MS/MS fragmentation spectra required for identification. Orbitrap analyzers scale performance with acquisition time and necessarily sacrifice sensitivity and resolving power to deliver higher acquisition rates. We developed a new mass spectrometer that combines a mass-resolving quadrupole, the Orbitrap, and the novel Asymmetric Track Lossless (Astral) analyzer. The new hybrid instrument enables faster acquisition of high-resolution accurate mass (HRAM) MS/MS spectra compared with state-of-the-art mass spectrometers. Accordingly, new proteomics methods were developed that leverage the strengths of each HRAM analyzer, whereby the Orbitrap analyzer performs full scans with a high dynamic range and resolution, synchronized with the Astral analyzer's acquisition of fast and sensitive HRAM MS/MS scans. Substantial improvements are demonstrated over previous methods using current state-of-the-art mass spectrometers

引用

页码：15656 / 15664

页数：9

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