Memory T Cells Discrepancies in COVID-19 Patients

被引：7
作者：
Al Saihati, Hajir A. [1 ]
Hussein, Hosni A. M. [2 ]
Thabet, Ali A. [3 ]
Wardany, Ahmed A. [2 ]
Mahmoud, Sabry Y. [4 ,5 ]
Farrag, Eman S. [1 ,6 ]
Mohamed, Taha I. A. [2 ]
Fathy, Samah M. [7 ]
Elnosary, Mohamed E. [8 ]
Sobhy, Ali [9 ]
Ahmed, Abdelazeem E. [9 ]
El-Adly, Ahmed M. [2 ]
El-Shenawy, Fareed S. [2 ]
Elsadek, Asmaa A. [10 ]
Rayan, Amal [11 ]
Zahran, Zeinab Albadry M. [12 ]
El-Badawy, Omnia [13 ]
El-Naggar, Mohamed G. M. [14 ]
Afifi, Magdy M. [8 ]
Zahran, Asmaa M. [14 ]
机构：
[1] Univ Hafr Al Batin, Coll Appl Med Sci, Dept Clin Lab Sci, POB 1803, Hafar Al Batin 31991, Saudi Arabia
[2] Al Azhar Univ, Fac Sci, Dept Bot & Microbiol, Assiut 71524, Egypt
[3] Al Azhar Univ, Fac Sci, Dept Zool, Assiut 71524, Egypt
[4] Univ Hafr Al Batin, Coll Sci, Biol Dept, Hafar al Batin 31991, Saudi Arabia
[5] Sohag Univ, Dept Microbiol, Sohag 82524, Egypt
[6] South Valley Univ, Dept Microbiol, Qena 83523, Egypt
[7] Fayoum Univ, Fac Sci, Dept Zool, Al Fayyum 63514, Egypt
[8] Al Azhar Univ, Fac Sci, Dept Bot & Microbiol, Nasr City 11884, Egypt
[9] Al Azhar Univ, Fac Med, Dept Clin Pathol, Assiut 71524, Egypt
[10] Chest Hosp, Dept Virol, Assiut 71514, Egypt
[11] Assiut Univ, Fac Med, Dept Clin Oncol, Assiut 71515, Egypt
[12] Assiut Univ, Fac Med, Dept Clin Pathol, Assiut 71515, Egypt
[13] Assiut Univ, Fac Med, Dept Med Microbiol & Immunol, Assiut 71515, Egypt
[14] Assiut Univ, South Egypt Canc Inst, Dept Clin Pathol, Assiut 71515, Egypt
来源：
MICROORGANISMS | 2023年 / 11卷 / 11期
关键词：
COVID-19; SARS-CoV-2; T cell subtypes; memory T cells; CORONAVIRUS; STEM;
D O I：
10.3390/microorganisms11112737
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The immune response implicated in Coronavirus disease 2019 (COVID-19) pathogenesis remains to be fully understood. The present study aimed to clarify the alterations in CD4+ and CD8+ memory T cells' compartments in SARS-CoV-2-infected patients, with an emphasis on various comorbidities affecting COVID-19 patients. Peripheral blood samples were collected from 35 COVID-19 patients, 16 recovered individuals, and 25 healthy controls, and analyzed using flow cytometry. Significant alterations were detected in the percentage of CD8+ T cells and effector memory-expressing CD45RA CD8+ T cells (TEMRA) in COVID-19 patients compared to healthy controls. Interestingly, altered percentages of CD4+ T cells, CD8+ T cells, T effector (TEff), T naive cells (TNs), T central memory (TCM), T effector memory (TEM), T stem cell memory (TSCM), and TEMRA T cells were significantly associated with the disease severity. Male patients had more CD8+ TSCMs and CD4+ TNs cells, while female patients had a significantly higher percentage of effector CD8+CD45RA+ T cells. Moreover, altered percentages of CD8+ TNs and memory CD8+CD45RO+ T cells were detected in diabetic and non-diabetic COVID-19 patients, respectively. In summary, this study identified alterations in memory T cells among COVID-19 patients, revealing a sex bias in the percentage of memory T cells. Moreover, COVID-19 severity and comorbidities have been linked to specific subsets of T memory cells which could be used as therapeutic, diagnostic, and protective targets for severe COVID-19.
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页数：14
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