Differential expression of immunoregulatory cytokines in adipose tissue and liver in response to high fat and high sugar diets in female mice

被引:3
作者
Weiner, Juliane [1 ]
Dommel, Sebastian [2 ,3 ]
Gebhardt, Claudia [2 ,3 ]
Hanschkow, Martha [4 ]
Popkova, Yulia [4 ]
Krause, Kerstin [1 ,5 ]
Kloeting, Nora [2 ,3 ]
Blueher, Matthias [1 ,2 ,3 ]
Schiller, Juergen [4 ]
Heiker, John T. [2 ,3 ,6 ]
机构
[1] Univ Leipzig, Med Dept Endocrinol Nephrol Rheumatol 3, Med Ctr, Leipzig, Germany
[2] Univ Leipzig, Helmholtz Inst Metab, Helmholtz Inst Metab Obes & Vasc Res HI MAG, Helmholtz Zentrum Munchen, Leipzig, Germany
[3] Univ Hosp Leipzig, Leipzig, Germany
[4] Univ Leipzig, Inst Med Phys & Biophys, Med Fac, Leipzig, Germany
[5] Deutsch Zent Diabetesforschung DZD E V, Neuherberg, Germany
[6] Univ Leipzig, Inst Biochem, Fac Life Sci, Leipzig, Germany
来源
FRONTIERS IN NUTRITION | 2023年 / 10卷
关键词
adipose tissue; obesity; inflammation; high fat diet; high sugar diet; lipid profile; nutrient; nutrition; TUMOR-NECROSIS-FACTOR; OBESITY; FRUCTOSE; INFLAMMATION; SUCROSE; ACID; LIPOGENESIS;
D O I
10.3389/fnut.2023.1275160
中图分类号
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号
100403 ;
摘要
A comprehensive understanding of how dietary components impact immunoregulatory gene expression in adipose tissue (AT) and liver, and their respective contributions to metabolic health in mice, remains limited. The current study aimed to investigate the metabolic consequences of a high-sucrose diet (HSD) and a high-fat diet (HFD) in female mice with a focus on differential lipid- and sucrose-induced changes in immunoregulatory gene expression in AT and liver. Female C57BL/6 J mice were fed a purified and macronutrient matched high fat, high sugar, or control diets for 12 weeks. Mice were extensively phenotyped, including glucose and insulin tolerance tests, adipose and liver gene and protein expression analysis by qPCR and Western blot, tissue lipid analyses, as well as histological analyses. Compared to the control diet, HSD- and HFD-fed mice had significantly higher body weights, with pronounced obesity along with glucose intolerance and insulin resistance only in HFD-fed mice. HSD-fed mice exhibited an intermediate phenotype, with mild metabolic deterioration at the end of the study. AT lipid composition was significantly altered by both diets, and inflammatory gene expression was only significantly induced in HFD-fed mice. In the liver however, histological analysis revealed that both HSD- and HFD-fed mice had pronounced ectopic lipid deposition indicating hepatic steatosis, but more pronounced in HSD-fed mice. This was in line with significant induction of pro-inflammatory gene expression specifically in livers of HSD-fed mice. Overall, our findings suggest that HFD consumption in female mice induces more profound inflammation in AT with pronounced deterioration of metabolic health, whereas HSD induced more pronounced hepatic steatosis and inflammation without yet affecting glucose metabolism.
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页数:12
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共 51 条
  • [1] Obesity: global epidemiology and pathogenesis
    Blueher, Matthias
    [J]. NATURE REVIEWS ENDOCRINOLOGY, 2019, 15 (05) : 288 - 298
  • [2] Adipose Tissue Dysfunction in Obesity
    Bluehre, M.
    [J]. EXPERIMENTAL AND CLINICAL ENDOCRINOLOGY & DIABETES, 2009, 117 (06) : 241 - 250
  • [3] CD36, a signaling receptor and fatty acid transporter that regulates immune cell metabolism and fate
    Chen, Yiliang
    Zhang, Jue
    Cui, Weiguo
    Silverstein, Roy L.
    [J]. JOURNAL OF EXPERIMENTAL MEDICINE, 2022, 219 (06)
  • [4] Dietary sugar restriction reduces hepatic de novo lipogenesis in adolescent boys with fatty liver disease
    Cohen, Catherine C.
    Li, Kelvin W.
    Alazraki, Adina L.
    Beysen, Carine
    Carrier, Carissa A.
    Cleeton, Rebecca L.
    Dandan, Mohamad
    Figueroa, Janet
    Knight-Scott, Jack
    Knott, Cynthia J.
    Newton, Kimberly P.
    Nyangau, Edna M.
    Sirlin, Claude B.
    Ugalde-Nicalo, Patricia A.
    Welsh, Jean A.
    Hellerstein, Marc K.
    Schwimmer, Jeffrey B.
    Vos, Miriam B.
    [J]. JOURNAL OF CLINICAL INVESTIGATION, 2021, 131 (24)
  • [5] High-Fat Diet Alters the Expression of Reference Genes in Male Mice
    Fan, Xiuqin
    Yao, Hongyang
    Liu, Xuanyi
    Shi, Qiaoyu
    Lv, Liang
    Li, Ping
    Wang, Rui
    Tang, Tiantian
    Qi, Kemin
    [J]. FRONTIERS IN NUTRITION, 2020, 7
  • [6] Adipokines in health and disease
    Fasshauer, Mathias
    Blueher, Matthias
    [J]. TRENDS IN PHARMACOLOGICAL SCIENCES, 2015, 36 (07) : 461 - 470
  • [7] Adiposoft: automated software for the analysis of white adipose tissue cellularity in histological sections
    Galarraga, Miguel
    Campion, Javier
    Munoz-Barrutia, Arrate
    Boque, Noemi
    Moreno, Haritz
    Alfredo Martinez, Jose
    Milagro, Fermin
    Ortiz-de-Solorzano, Carlos
    [J]. JOURNAL OF LIPID RESEARCH, 2012, 53 (12) : 2791 - 2796
  • [8] Fatty Acid Synthase Cooperates with Glyoxalase 1 to Protect against Sugar Toxicity
    Garrido, Damien
    Rubin, Thomas
    Poidevin, Mickael
    Maroni, Brigitte
    Le Rouzic, Arnaud
    Parvy, Jean-Philippe
    Montagne, Jacques
    [J]. PLOS GENETICS, 2015, 11 (02): : 1 - 26
  • [9] Downregulation of de Novo Fatty Acid Synthesis in Subcutaneous Adipose Tissue of Moderately Obese Women
    Guiu-Jurado, Esther
    Auguet, Teresa
    Berlanga, Alba
    Aragones, Gemma
    Aguilar, Carmen
    Sabench, Fatima
    Armengol, Sandra
    Antonio Porras, Jose
    Marti, Andreu
    Jorba, Rosa
    Hernandez, Merce
    del Castillo, Daniel
    Richart, Cristobal
    [J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2015, 16 (12) : 29911 - 29922
  • [10] Comparative Effects of Fructose and Glucose on Lipogenic Gene Expression and Intermediary Metabolism in HepG2 Liver Cells
    Hirahatake, Kristin M.
    Meissen, John K.
    Fiehn, Oliver
    Adams, Sean H.
    [J]. PLOS ONE, 2011, 6 (11):