Analysis and purification of innate lymphoid cells in human intestine and blood by flow cytometry

被引：1
作者：
Qi, Jingjing [1 ,2 ]
Hong, Liwen [3 ]
Wang, Zhengting [3 ]
Liu, Yingbin [1 ,2 ]
Shen, Lei [1 ,2 ]
Su, Bing [1 ,2 ,3 ]
机构：
[1] Shanghai Jiao Tong Univ, Sch Med, Dept Immunol & Microbiol, Shanghai Inst Immunol, Shanghai 200025, Peoples R China
[2] Shanghai Jiao Tong Univ, Renji Hosp, Shanghai Inst Canc Biol, Sch Med, Shanghai 200025, Peoples R China
[3] Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp, Dept Gastroenterol, Shanghai 200025, Peoples R China
来源：
STAR PROTOCOLS | 2023年 / 4卷 / 02期
基金：
中国博士后科学基金; 中国国家自然科学基金;
关键词：
Cancer; Flow Cytometry/Mass Cytometry; Health Sciences; Immunology; Single Cell;
D O I：
10.1016/j.xpro.2023.102180
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The role of innate lymphoid cells (ILCs)-including natural killer cells, helper-like ILC1s, ILC2s, ILC3s, and lymphoid tissue inducers-in human cancer is still poorly understood due to the scarcity of cell number. To address this, we present a protocol to analyze or purify ILCs from human blood, adjacent intestine, and colorectal tumor tissue. We describe steps for tissue and blood treatment, density centrifugation, antibody staining, and cell sorting. For complete details on the use and execution of this protocol, please refer to Qi et al. (2021).1
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页数：9
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