Rat bone marrow mesenchymal stem cells induced by rrPDGF-BB promotes bone regeneration during distraction osteogenesis

被引:7
作者
Wu, Shuo [1 ]
Zhang, Lijie [2 ]
Zhang, Ruidan [3 ]
Yang, Kang [4 ]
Wei, Qin [5 ]
Jia, Qiyu [1 ]
Guo, Jian [1 ]
Ma, Chuang [1 ]
机构
[1] Xinjiang Med Univ, Dept Microrepair & Reconstruct, Affiliated Hosp 1, Urumqi, Peoples R China
[2] Xinjiang Med Univ, Dept Neurol, Affiliated Hosp 2, Urumqi, Peoples R China
[3] Guangdong New Omega Med Ctr, Guangzhou, Peoples R China
[4] Hand & Foot Microsurg Third Peoples Hosp Xinjiang, Urumqi, Peoples R China
[5] Xinjiang Med Univ, Anim Expt Ctr, Urumqi, Peoples R China
基金
中国国家自然科学基金;
关键词
BMSCs; distraction osteogenesis (DO); PDGF-BB; rat; osteogenesis; femur; IN-VIVO; THERAPY;
D O I
10.3389/fbioe.2023.1110703
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Background: In the clinical treatment of large bone defects, distraction osteogenesis can be used. However, some patients may suffer from poor bone regeneration, or even delayed healing or non-union. Problems with the aggregation and proliferation of primary osteoblasts, or problems with the differentiation of primary osteoblasts will lead to poor bone regeneration. Therefore, supplementing exogenous primary osteoblasts and growth factors when using distraction osteogenesis may be a treatment plan with great potential.Methods: Bone marrow mesenchymal stem cells (BMSCs) were extracted from rats and cultured. Subsequently, Recombinant Rat Platelet-derived Growth Factor BB (rrPDGF-BB) was used to induce bone marrow mesenchymal stem cells. At the same time, male adult rats were selected to make the right femoral distraction osteogenesis model. During the mineralization period, phosphate buffer salt solution (control group), non-induction bone marrow mesenchymal stem cells (group 1) and recombinant rat platelet-derived growth factor BB intervened bone marrow mesenchymal stem cells (group 2) were injected into the distraction areas of each group. Then, the experimental results were evaluated with imaging and histology. Statistical analysis of the data showed that the difference was statistically significant if p < 0.05.Results: After intervention with recombinant rat platelet-derived growth factor BB on bone marrow mesenchymal stem cells, the cell morphology changed into a thin strip. After the cells were injected in the mineralization period, the samples showed that the callus in group 2 had greater hardness and the color close to the normal bone tissue; X-ray examination showed that there were more new callus in the distraction space of group 2; Micro-CT examination showed that there were more new bone tissues in group 2; Micro-CT data at week eight showed that the tissue volume, bone volume, percent bone volume, bone trabecular thickness, bone trabecular number and bone mineral density in group 2 were the largest, and the bone trabecular separation in group 2 was the smallest. There was a statistical difference between the groups (p < 0.05); HE staining confirmed that group 2 formed more blood vessels and chondrocytes earlier than the control group. At 8 weeks, the bone marrow cavity of group 2 was obvious, and some of them had been fused.Conclusion: The study confirmed that injecting bone marrow mesenchymal stem cellsBB into the distraction space of rats can promote the formation of new bone in the distraction area and promote the healing of distraction osteogenesis.
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页数:13
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共 57 条
  • [31] Cyclic Distraction-Compression Dynamization Technique Enhances the Bone Formation During Distraction Osteogenesis
    Liu, Yanshi
    Cai, Feiyu
    Liu, Kai
    Liu, Jialin
    Zhang, Xiaoxu
    Yusufu, Aihemaitijiang
    [J]. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, 2022, 9
  • [32] Effect of recombinant PDGF-BB on bone formation in the presence of β-tricalcium phosphate and bovine bone mineral matrix: a pilot study in rat calvarial defects
    Luvizuto, Eloa R.
    Tangl, Stefan
    Dobsak, Toni
    Reich, Karoline
    Gruber, Reinhard
    Sonoda, Celso K.
    Okamoto, Roberta
    [J]. BMC ORAL HEALTH, 2016, 16 : 1 - 7
  • [33] Optimization of decalcification techniques for histologic examination of the rat maxillary and mandibular incisors for toxicity studies
    Marinopoulos, Anastasia E.
    Ayres, Samuel C.
    Biswas, Sabyasachi
    Huang, Xin
    Mantena, Srinivasa R.
    Peterson, Richard A., II
    Fossey, Stacey L.
    [J]. JOURNAL OF HISTOTECHNOLOGY, 2022, 45 (01) : 2 - 9
  • [34] Sclerostin antibody enhances bone formation in a rat model of distraction osteogenesis
    McDonald, Michelle M.
    Morse, Alyson
    Birke, Oliver
    Yu, Nicole Y. C.
    Mikulec, Kathy
    Peacock, Lauren
    Schindeler, Aaron
    Liu, Min
    Ke, Hua Z.
    Little, David G.
    [J]. JOURNAL OF ORTHOPAEDIC RESEARCH, 2018, 36 (04) : 1106 - 1113
  • [35] Mi J, 2021, TISSUE ENG PT A, V27, P87, DOI [10.1089/ten.tea.2020.0009, 10.1089/ten.TEA.2020.0009]
  • [36] Strategies for large bone defect reconstruction after trauma, infections or tumour excision: a comprehensive review of the literature
    Migliorini, Filippo
    La Padula, Gerardo
    Torsiello, Ernesto
    Spiezia, Filippo
    Oliva, Francesco
    Maffulli, Nicola
    [J]. EUROPEAN JOURNAL OF MEDICAL RESEARCH, 2021, 26 (01)
  • [37] Recombinant Human Platelet-Derived Growth Factor-BB Augmentation of New-Bone Formation in a Rat Model of Distraction Osteogenesis
    Moore, Douglas C.
    Ehrlich, Michael G.
    McAllister, Scott C.
    Machan, Jason T.
    Hart, Charles E.
    Voigt, Clifford
    Lesieur-Brooks, Anne M.
    Weber, Elizabeth W.
    [J]. JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME, 2009, 91A (08) : 1973 - 1984
  • [38] Novel polychrome staining distinguishing osteochondral tissue and bone cells in decalcified paraffin sections
    Nakamura, Teppei
    Sumi, Kanako
    Tsuji, Erika
    Hosotani, Marina
    Namba, Takashi
    Ichii, Osamu
    Irie, Takao
    Nagasaki, Ken-ichi
    Kon, Yasuhiro
    Mishima, Takashi
    Yoshiyasu, Tomoji
    [J]. CELL AND TISSUE RESEARCH, 2021, 385 (03) : 727 - 737
  • [39] Identification of Functionally Distinct Mx1+αSMA plus Periosteal Skeletal Stem Cells
    Ortinau, Laura C.
    Wang, Hamilton
    Lei, Kevin
    Deveza, Lorenzo
    Jeong, Youngjae
    Hara, Yannis
    Grafe, Ingo
    Rosenfeld, Scott B.
    Lee, Dongjun
    Lee, Brendan
    Scadden, David T.
    Park, Dongsu
    [J]. CELL STEM CELL, 2019, 25 (06) : 784 - +
  • [40] Fundamentals of bone vasculature: Specialization, interactions and functions
    Owen-Woods, Charlotte
    Kusumbe, Anjali
    [J]. SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY, 2022, 123 : 36 - 47