Rat bone marrow mesenchymal stem cells induced by rrPDGF-BB promotes bone regeneration during distraction osteogenesis

被引:7
作者
Wu, Shuo [1 ]
Zhang, Lijie [2 ]
Zhang, Ruidan [3 ]
Yang, Kang [4 ]
Wei, Qin [5 ]
Jia, Qiyu [1 ]
Guo, Jian [1 ]
Ma, Chuang [1 ]
机构
[1] Xinjiang Med Univ, Dept Microrepair & Reconstruct, Affiliated Hosp 1, Urumqi, Peoples R China
[2] Xinjiang Med Univ, Dept Neurol, Affiliated Hosp 2, Urumqi, Peoples R China
[3] Guangdong New Omega Med Ctr, Guangzhou, Peoples R China
[4] Hand & Foot Microsurg Third Peoples Hosp Xinjiang, Urumqi, Peoples R China
[5] Xinjiang Med Univ, Anim Expt Ctr, Urumqi, Peoples R China
基金
中国国家自然科学基金;
关键词
BMSCs; distraction osteogenesis (DO); PDGF-BB; rat; osteogenesis; femur; IN-VIVO; THERAPY;
D O I
10.3389/fbioe.2023.1110703
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Background: In the clinical treatment of large bone defects, distraction osteogenesis can be used. However, some patients may suffer from poor bone regeneration, or even delayed healing or non-union. Problems with the aggregation and proliferation of primary osteoblasts, or problems with the differentiation of primary osteoblasts will lead to poor bone regeneration. Therefore, supplementing exogenous primary osteoblasts and growth factors when using distraction osteogenesis may be a treatment plan with great potential.Methods: Bone marrow mesenchymal stem cells (BMSCs) were extracted from rats and cultured. Subsequently, Recombinant Rat Platelet-derived Growth Factor BB (rrPDGF-BB) was used to induce bone marrow mesenchymal stem cells. At the same time, male adult rats were selected to make the right femoral distraction osteogenesis model. During the mineralization period, phosphate buffer salt solution (control group), non-induction bone marrow mesenchymal stem cells (group 1) and recombinant rat platelet-derived growth factor BB intervened bone marrow mesenchymal stem cells (group 2) were injected into the distraction areas of each group. Then, the experimental results were evaluated with imaging and histology. Statistical analysis of the data showed that the difference was statistically significant if p < 0.05.Results: After intervention with recombinant rat platelet-derived growth factor BB on bone marrow mesenchymal stem cells, the cell morphology changed into a thin strip. After the cells were injected in the mineralization period, the samples showed that the callus in group 2 had greater hardness and the color close to the normal bone tissue; X-ray examination showed that there were more new callus in the distraction space of group 2; Micro-CT examination showed that there were more new bone tissues in group 2; Micro-CT data at week eight showed that the tissue volume, bone volume, percent bone volume, bone trabecular thickness, bone trabecular number and bone mineral density in group 2 were the largest, and the bone trabecular separation in group 2 was the smallest. There was a statistical difference between the groups (p < 0.05); HE staining confirmed that group 2 formed more blood vessels and chondrocytes earlier than the control group. At 8 weeks, the bone marrow cavity of group 2 was obvious, and some of them had been fused.Conclusion: The study confirmed that injecting bone marrow mesenchymal stem cellsBB into the distraction space of rats can promote the formation of new bone in the distraction area and promote the healing of distraction osteogenesis.
引用
收藏
页数:13
相关论文
共 57 条
  • [21] Homsi Jade, 2007, Cancer Control, V14, P285
  • [22] Exosomes Secreted by Young Mesenchymal Stem Cells Promote New Bone Formation During Distraction Osteogenesis in Older Rats
    Jia, Yachao
    Qiu, Shuo
    Xu, Jia
    Kang, Qinglin
    Chai, Yimin
    [J]. CALCIFIED TISSUE INTERNATIONAL, 2020, 106 (05) : 509 - 517
  • [23] Jiang T., 2021, STEM CELL RES THER, V25, P1976, DOI [10.3969/j.issn.2095-4344.2188, DOI 10.3969/J.ISSN.2095-4344.2188]
  • [24] Jiang T., 2021, CHIN J TISSUE ENG RE, V25, P3937, DOI [10.12307/2021.001, DOI 10.12307/2021.001]
  • [25] Mesenchymal Stem Cell Therapy for Bone Regeneration
    Jin, Yuan-Zhe
    Lee, Jae Hyup
    [J]. CLINICS IN ORTHOPEDIC SURGERY, 2018, 10 (03) : 271 - 278
  • [26] Platelet-derived growth factor-BB regenerates functional periodontal ligament in the tooth replantation
    Komatsu, Koichiro
    Ideno, Hisashi
    Shibata, Tatsuya
    Nakashima, Kazuhisa
    Nifuji, Akira
    [J]. SCIENTIFIC REPORTS, 2022, 12 (01)
  • [27] Mitogenesis of Vascular Smooth Muscle Cell Stimulated by Platelet-Derived Growth Factor-bb Is Inhibited by Blocking of Intracellular Signaling by Epigallocatechin-3-O-Gallate
    Lee, Mi Hee
    Kwon, Byeong-Ju
    Koo, Min-Ah
    You, Kyung Eun
    Park, Jong-Chul
    [J]. OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, 2013, 2013
  • [28] Biodegradable magnesium combined with distraction osteogenesis synergistically stimulates bone tissue regeneration via CGRP-FAK-VEGF signaling axis
    Li, Ye
    Xu, Jiankun
    Mi, Jie
    He, Xuan
    Pan, Qi
    Zheng, Lizhen
    Zu, Haiyue
    Chen, Ziyi
    Dai, Bingyang
    Li, Xu
    Pang, Qianqian
    Zou, Li
    Zhou, Liangbin
    Huang, Le
    Tong, Wenxue
    Li, Gang
    Qin, Ling
    [J]. BIOMATERIALS, 2021, 275
  • [29] Moringa oleifera leaf extracts protect BMSC osteogenic induction following peroxidative damage by activating the PI3K/Akt/Foxo1 pathway
    Liu, Meiling
    Ding, Haifeng
    Wang, Hongzhi
    Wang, Manfeng
    Wu, Xiaowei
    Gan, Lu
    Cheng, Luyang
    Li, Xianglu
    [J]. JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH, 2021, 16 (01)
  • [30] Hypoxia During the Consolidation Phase of Distraction Osteogenesis Promotes Bone Regeneration
    Liu, Yanshi
    Liu, Jialin
    Cai, Feiyu
    Liu, Kai
    Zhang, Xiaoxu
    Yusufu, Aihemaitijiang
    [J]. FRONTIERS IN PHYSIOLOGY, 2022, 13